Acute physical exercise reverses S-nitrosation of the insulin receptor, insulin receptor substrate 1 and protein kinase B/Akt in diet-induced obese Wistar rats

dc.creatorPauli, JR
dc.creatorRopelle, ER
dc.creatorCintra, DE
dc.creatorCarvalho, MA
dc.creatorMoraes, JC
dc.creatorDe Souza, CT
dc.creatorVelloso, LA
dc.creatorCarvalheira, JBC
dc.creatorSaad, MJA
dc.date2008
dc.date42005
dc.date2014-07-30T13:43:37Z
dc.date2015-11-26T18:06:33Z
dc.date2014-07-30T13:43:37Z
dc.date2015-11-26T18:06:33Z
dc.date.accessioned2018-03-29T00:48:46Z
dc.date.available2018-03-29T00:48:46Z
dc.identifierJournal Of Physiology-london. Blackwell Publishing, v. 586, n. 2, n. 659, n. 671, 2008.
dc.identifier0022-3751
dc.identifierWOS:000252678800028
dc.identifier10.1113/jphysiol.2007.142414
dc.identifierhttp://www.repositorio.unicamp.br/jspui/handle/REPOSIP/54216
dc.identifierhttp://repositorio.unicamp.br/jspui/handle/REPOSIP/54216
dc.identifier.urihttp://repositorioslatinoamericanos.uchile.cl/handle/2250/1293393
dc.descriptionEarly evidence demonstrates that exogenous nitric oxide (NO) and the NO produced by inducible nitric oxide synthase (iNOS) can induce insulin resistance. Here, we investigated whether this insulin resistance, mediated by S-nitrosation of proteins involved in early steps of the insulin signal transduction pathway, could be reversed by acute physical exercise. Rats on a high-fat diet were subjected to swimming for two 3 h-longbouts, separated by a 45 min rest period. Two or 16 h after the exercise protocol the rats were killed and proteins from the insulin signalling pathway were analysed by immunoprecipitation and immunoblotting. We demonstrated that a high-fat diet led to an increase in the iNOS protein level and S-nitrosation of insulin receptor beta(IR beta), insulin receptor substrate 1 (IRS1) and Akt. Interestingly, an acute bout of exercise reduced iNOS expression and S-nitrosation of proteins involved in the early steps of insulin action, and improved insulin sensitivity in diet-induced obesity rats. Furthermore, administration of GSNO (NO donor) prevents this improvement in insulin action and the use of an inhibitor of iNOS (L-N-6-(1-iminoethyl)lysine; L-NIL) simulates the effects of exercise on insulin action, insulin signalling and S-nitrosation of IR beta, IRS1 and Akt. In summary, a single bout of exercise reverses insulin sensitivity in diet-induced obese rats by improving the insulin signalling pathway, in parallel with a decrease in iNOS expression and in the S-nitrosation of IR/IRS1/Akt. The decrease in iNOS protein expression in the muscle of diet-induced obese rats after an acute bout of exercise was accompanied by an increase in AMP-activated protein kinase (AMPK) activity. These results provide new insights into the mechanism by which exercise restores insulin sensitivity.
dc.description586
dc.description2
dc.description659
dc.description671
dc.languageen
dc.publisherBlackwell Publishing
dc.publisherOxford
dc.publisherInglaterra
dc.relationJournal Of Physiology-london
dc.relationJ. Physiol.-London
dc.rightsfechado
dc.sourceWeb of Science
dc.subjectNitric-oxide Synthase
dc.subjectSkeletal-muscle
dc.subjectGlucose-transport
dc.subjectTargeted Disruption
dc.subjectSignal-transduction
dc.subjectAdipose-tissue
dc.subjectSingle Bout
dc.subjectResistance
dc.subjectExpression
dc.subjectSensitivity
dc.titleAcute physical exercise reverses S-nitrosation of the insulin receptor, insulin receptor substrate 1 and protein kinase B/Akt in diet-induced obese Wistar rats
dc.typeArtículos de revistas


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