Modelling, simulation and factorial design as tools for the in silico process optimisation of fructooligosaccharide production by immobilised fructosyltransferase in a basket reactor

dc.creatorSoares, MBAH
dc.creatorRodrigues, MI
dc.creatorMaugeri, F
dc.date2014
dc.dateJAN-FEB
dc.date2014-08-01T18:42:36Z
dc.date2015-11-26T18:06:28Z
dc.date2014-08-01T18:42:36Z
dc.date2015-11-26T18:06:28Z
dc.date.accessioned2018-03-29T00:48:41Z
dc.date.available2018-03-29T00:48:41Z
dc.identifierBiocatalysis And Biotransformation. Informa Healthcare, v. 32, n. 1, n. 84, n. 92, 2014.
dc.identifier1024-2422
dc.identifier1029-2446
dc.identifierWOS:000333520100011
dc.identifier10.3109/10242422.2013.873794
dc.identifierhttp://www.repositorio.unicamp.br/jspui/handle/REPOSIP/82346
dc.identifierhttp://repositorio.unicamp.br/jspui/handle/REPOSIP/82346
dc.identifier.urihttp://repositorioslatinoamericanos.uchile.cl/handle/2250/1293368
dc.descriptionFundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)
dc.descriptionModelling, simulation and optimisation of the production of fructooligosaccharides (FOS) by fructosyltransferase immobilised on inorganic niobium support and applied in a basket reactor (BR) were used for in silico optimisation of the process. Simulink and MATLAATLAB 6.0 were used as computational tools. The kinetic process data were obtained experimentally using purified fructosyltransferase produced by Rhodotula sp. Optimisation experiments were carried out using simulation, according to the factorial design methodology, using a two central composite rotatable design. In the first design, the variables with the highest influence were determined; in the second design, the conditions to produce FOS were optimised. The optimum conditions were 14 Ui/ml for the activity of enzyme, immobilised in a niobium ore, and a stirring speed of 45 rpm. Under these conditions, FOS yield was 50.6% after 24 h of synthesis, at 50 C and pH 4.5. The results demonstrated the good efficiency of the BR, as well as showing that the film mass transfer coefficient around the biocatalyst has a significant influence during the reaction and production of FOS.
dc.description32
dc.description1
dc.description84
dc.description92
dc.descriptionFundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)
dc.descriptionFundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)
dc.languageen
dc.publisherInforma Healthcare
dc.publisherLondon
dc.publisherInglaterra
dc.relationBiocatalysis And Biotransformation
dc.relationBiocatal. Biotransform.
dc.rightsfechado
dc.rightshttp://informahealthcare.com/userimages/ContentEditor/1255620309227/Copyright_And_Permissions.pdf
dc.sourceWeb of Science
dc.subjectFructosyltransferase
dc.subjectRhodotorula sp.
dc.subjectfructooligosaccharides
dc.subjectimmobilised enzyme
dc.subjectbasket reactor
dc.subjectmathematical modelling
dc.subjectsimulation and process optimisation
dc.subjectFructo-oligosaccharides
dc.subjectAspergillus-japonicus
dc.subjectEnzymatic Production
dc.subjectMathematical-model
dc.subjectSucrose
dc.titleModelling, simulation and factorial design as tools for the in silico process optimisation of fructooligosaccharide production by immobilised fructosyltransferase in a basket reactor
dc.typeArtículos de revistas


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