Effects of Sucrose on the Extracellular Matrix of Plaque-Like Biofilm Formed in vivo, Studied by Proteomic Analysis

dc.creatorLeme, AFP
dc.creatorBellato, CM
dc.creatorBedi, G
dc.creatorCury, AADB
dc.creatorKoo, H
dc.creatorCury, JA
dc.date2008
dc.date2014-07-30T17:21:47Z
dc.date2015-11-26T18:06:26Z
dc.date2014-07-30T17:21:47Z
dc.date2015-11-26T18:06:26Z
dc.date.accessioned2018-03-29T00:48:36Z
dc.date.available2018-03-29T00:48:36Z
dc.identifierCaries Research. Karger, v. 42, n. 6, n. 435, n. 443, 2008.
dc.identifier0008-6568
dc.identifierWOS:000261132700004
dc.identifier10.1159/000159607
dc.identifierhttp://www.repositorio.unicamp.br/jspui/handle/REPOSIP/65104
dc.identifierhttp://repositorio.unicamp.br/jspui/handle/REPOSIP/65104
dc.identifier.urihttp://repositorioslatinoamericanos.uchile.cl/handle/2250/1293353
dc.descriptionFundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)
dc.descriptionConselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)
dc.descriptionPrevious studies have shown that sucrose promotes changes in the composition of the extracellular matrix (ECM) of plaque-like biofilm (PLB), but its effect on protein expression has not been studied in vivo. Therefore, the protein compositions of ECM of PLB formed with and without sucrose exposure were analyzed by two-dimensional gel electrophoresis and matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF MS). For this purpose, a crossover study was conducted during two phases of 14 days each, during which a volunteer wore a palatal appliance containing eight enamel blocks for PLB accumulation. In each phase, a 20% sucrose solution or distilled and deionized water (control) were extraorally dripped onto the blocks 8x/day. On the 14th day, the PLB were collected, the ECM proteins were extracted, separated by two-dimensional gel electrophoresis, digested by in-gel trypsin and MALDI-TOF MS analyzed. In the ECM of PLB formed under sucrose exposure, the following changes compared with the control PLB were observed: (1) the presence of upregulated proteins that may be involved in bacterial response to environmental changes induced by sucrose and (2) the absence of calcium binding proteins that may partly explain the low inorganic concentration found in ECM of PLB formed under sucrose exposure. The findings showing that sucrose affected the ECM protein composition of PLB in vivo provide further insight into the unique cariogenic properties of this dietary carbohydrate. Copyright (C) 2008 S. Karger AG, Basel
dc.description42
dc.description6
dc.description435
dc.description443
dc.descriptionFundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)
dc.descriptionConselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)
dc.descriptionNIH [RR14682]
dc.descriptionFaculty of Dentistry of Piracicaba, UNICAMP, SP, Brazil
dc.descriptionFundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)
dc.descriptionConselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)
dc.descriptionFAPESP [99/07185-7, 02/00293-3, 03/01536-0]
dc.descriptionCNPq [472392/03-4]
dc.descriptionNIH [RR14682]
dc.languageen
dc.publisherKarger
dc.publisherBasel
dc.publisherSuíça
dc.relationCaries Research
dc.relationCaries Res.
dc.rightsfechado
dc.rightshttp://www.karger.com/Services/RightsPermissions
dc.sourceWeb of Science
dc.subjectDental plaque
dc.subjectGel electrophoresis, two-dimensional
dc.subjectMass spectrometry
dc.subjectSucrose
dc.subjectStreptococcus-mutans
dc.subjectDental Biofilm
dc.subjectEnamel Demineralization
dc.subjectProtein Expression
dc.subjectLow Ph
dc.subjectSurface
dc.subjectSitu
dc.subjectStress
dc.subjectIdentification
dc.subjectPhosphatase
dc.titleEffects of Sucrose on the Extracellular Matrix of Plaque-Like Biofilm Formed in vivo, Studied by Proteomic Analysis
dc.typeArtículos de revistas


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