Effects of ulipristal acetate on sperm DNA fragmentation during in vitro incubation

dc.creatorMunuce, MJ
dc.creatorCicare, J
dc.creatorZumoffen, C
dc.creatorCaille, A
dc.creatorGhersevich, S
dc.creatorBahamondes, L
dc.date2013
dc.dateOCT
dc.date2014-08-01T18:29:56Z
dc.date2015-11-26T18:05:07Z
dc.date2014-08-01T18:29:56Z
dc.date2015-11-26T18:05:07Z
dc.date.accessioned2018-03-29T00:47:23Z
dc.date.available2018-03-29T00:47:23Z
dc.identifierEuropean Journal Of Contraception And Reproductive Health Care. Informa Healthcare, v. 18, n. 5, n. 355, n. 363, 2013.
dc.identifier1362-5187
dc.identifierWOS:000324618100005
dc.identifier10.3109/13625187.2013.813930
dc.identifierhttp://www.repositorio.unicamp.br/jspui/handle/REPOSIP/79779
dc.identifierhttp://repositorio.unicamp.br/jspui/handle/REPOSIP/79779
dc.identifier.urihttp://repositorioslatinoamericanos.uchile.cl/handle/2250/1293050
dc.descriptionConselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)
dc.descriptionObjective Ulipristal acetate (UPA) acts as an emergency contraceptive by inhibiting ovulation. This study explores possible additional effects on the fragmentation of sperm DNA during in vitro incubation. Methods Motile spermatozoa from healthy donors were selected by swim-up and incubated under capacitating conditions in control medium or with UPA (1, 10, 100, 1,000 or 10,000 ng/ml). In some experiments, 200 mu M of H2O2 were added to induce oxidative stress. The sperm chromatin dispersion test was performed to analyse DNA integrity (400 cells; 1000X). Lipid peroxidation (thiobarbituric acid assay), induced-acrosome reaction (AR) and sperm vitality (Eosin Y) were also evaluated in spermatozoa exposed to UPA and/or H2O2. Results During sperm incubation, the percentage of fragmented DNA increased significantly, from 15.0 +/- 1.3 to 41.0 +/- 4.5% (p < 0.001). In the presence of UPA, DNA fragmentation decreased significantly (p < 0.05), in a dose-dependent manner. At 100 and 1000 ng/ml, UPA also counteracted the effect of H2O2 and prevented DNA fragmentation. No effect on sperm vitality, lipid peroxidation or induced-AR was found with any treatment. Conclusions During in vitro sperm capacitation DNA fragmentation increased but the latter was counteracted in the presence of UPA, which possibly acted as a scavenger of reactive oxygen species produced by spermatozoa.
dc.description18
dc.description5
dc.description355
dc.description363
dc.descriptionConselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)
dc.descriptionConselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)
dc.descriptionCNPq [573747/2008-3]
dc.languageen
dc.publisherInforma Healthcare
dc.publisherLondon
dc.publisherInglaterra
dc.relationEuropean Journal Of Contraception And Reproductive Health Care
dc.relationEur. J. Contracept. Reprod. Health Care
dc.rightsfechado
dc.rightshttp://informahealthcare.com/userimages/ContentEditor/1255620309227/Copyright_And_Permissions.pdf
dc.sourceWeb of Science
dc.subjectSpermatozoa
dc.subjectUlipristal acetate
dc.subjectSperm chromatin dispersion test
dc.subjectDNA fragmentation
dc.subjectHuman-spermatozoa
dc.subjectProgesterone-receptor
dc.subjectHydrogen-peroxide
dc.subjectMale-infertility
dc.subjectLipid-peroxidation
dc.subjectAcrosome Reaction
dc.subjectOxidative Stress
dc.subjectSuperoxide Anion
dc.subjectCapacitation
dc.subjectFertilization
dc.titleEffects of ulipristal acetate on sperm DNA fragmentation during in vitro incubation
dc.typeArtículos de revistas


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