Inflammatory and bone-related genes are modulated by aging in human periodontal ligament cells

dc.creatorBenatti, BB
dc.creatorSilverio, KG
dc.creatorCasati, MZ
dc.creatorSallum, EA
dc.creatorNociti, FH
dc.date2009
dc.dateMAY
dc.date2014-11-19T15:28:08Z
dc.date2015-11-26T18:04:06Z
dc.date2014-11-19T15:28:08Z
dc.date2015-11-26T18:04:06Z
dc.date.accessioned2018-03-29T00:46:09Z
dc.date.available2018-03-29T00:46:09Z
dc.identifierCytokine. Academic Press Ltd- Elsevier Science Ltd, v. 46, n. 2, n. 176, n. 181, 2009.
dc.identifier1043-4666
dc.identifierWOS:000266346800005
dc.identifier10.1016/j.cyto.2009.01.002
dc.identifierhttp://www.repositorio.unicamp.br/jspui/handle/REPOSIP/60156
dc.identifierhttp://www.repositorio.unicamp.br/handle/REPOSIP/60156
dc.identifierhttp://repositorio.unicamp.br/jspui/handle/REPOSIP/60156
dc.identifier.urihttp://repositorioslatinoamericanos.uchile.cl/handle/2250/1292746
dc.descriptionConselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)
dc.descriptionPeriodontal ligament cells (PDLC) play a major role in periodontal tissues homeostasis and destruction. Most age-associated diseases seem to be closely related to an underlying chronic inflammatory state. Thus, the present study aimed at evaluating in PDLC the effect of aging on the basal levels of inflammatory and bone-related genes. Primary PDLC cultures were obtained from subjects aged 15-20 years (control- n = 5), and subjects aged more than 60 years (test- n = 5). Proliferation, cell viability and total secreted protein assays were performed, and mRNA levels were quantitatively assessed for interleukin (IL)-I beta, IL-4, IL-6 and IL-8, and for receptor activator of nuclear factor-kappa B ligand (RANKL) and osteoprotegerin (OPG) by real time PCR. Data analysis demonstrated that aging negatively influenced cell proliferation, whereas cell viability and total secreted protein were not affected (p > 0.05). Gene expression analysis showed that mRNA levels for RANKL and IL-8 were not affected by aging (p > 0.05) whereas, mRNA levels for IL-4 was significantly lower in aged cells (p < 0.05) and OPG, IL-1 beta and IL-6 mRNA levels were higher (p < 0.05). Data analysis suggests that aging decreased the ability of PDLC to proliferate and modulated the expression of important inflammatory and bone-related genes in periodontal ligament cells, favoring a proinflammatory and an antiresorptive profile. (C) 2009 Elsevier Ltd. All rights reserved.
dc.description46
dc.description2
dc.description176
dc.description181
dc.descriptionSao Paulo State Foundation
dc.descriptionConselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)
dc.descriptionConselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)
dc.languageen
dc.publisherAcademic Press Ltd- Elsevier Science Ltd
dc.publisherLondon
dc.publisherInglaterra
dc.relationCytokine
dc.relationCytokine
dc.rightsfechado
dc.rightshttp://www.elsevier.com/about/open-access/open-access-policies/article-posting-policy
dc.sourceWeb of Science
dc.subjectAging
dc.subjectPeriodontal ligament
dc.subjectCells
dc.subjectGene expression
dc.subjectKappa-b Ligand
dc.subjectReceptor Activator
dc.subjectOsteoclast Differentiation
dc.subjectProtein-synthesis
dc.subjectInhibitory Factor
dc.subjectOsteoprotegerin
dc.subjectFibroblasts
dc.subjectExpression
dc.subjectInterleukin-1-beta
dc.subjectMechanisms
dc.titleInflammatory and bone-related genes are modulated by aging in human periodontal ligament cells
dc.typeArtículos de revistas


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