Amperometric biosensor for ethanol based on co-immobilization of alcohol dehydrogenase and Meldola's Blue on multi-wall carbon nanotube

dc.creatorSantos, AS
dc.creatorPereira, AC
dc.creatorDuran, N
dc.creatorKubota, LT
dc.date2006
dc.dateOCT 5
dc.date2014-11-19T14:30:57Z
dc.date2015-11-26T18:03:50Z
dc.date2014-11-19T14:30:57Z
dc.date2015-11-26T18:03:50Z
dc.date.accessioned2018-03-29T00:45:50Z
dc.date.available2018-03-29T00:45:50Z
dc.identifierElectrochimica Acta. Pergamon-elsevier Science Ltd, v. 52, n. 1, n. 215, n. 220, 2006.
dc.identifier0013-4686
dc.identifierWOS:000241253100027
dc.identifier10.1016/j.electacta.2006.04.060
dc.identifierhttp://www.repositorio.unicamp.br/jspui/handle/REPOSIP/54250
dc.identifierhttp://www.repositorio.unicamp.br/handle/REPOSIP/54250
dc.identifierhttp://repositorio.unicamp.br/jspui/handle/REPOSIP/54250
dc.identifier.urihttp://repositorioslatinoamericanos.uchile.cl/handle/2250/1292666
dc.descriptionIn this work, multi-wall carbon nanotube (MWCT) is evaluated as transducer, stabilizer and immobilization matrix for the construction of amperometric biosensor based on alcohol dehydrogenase (ADH) and Meldola's Blue (MB). The amperometric response was based on the electro catalytic properties of MB to oxidize NADH, which was generated in the enzymatic reaction of ethanol with NAD(+) under catalysis of ADH. It is shown that the employed materials are promising as electrochemical mediators and enzyme stabilizers. The enzyme was immobilized onto the MWCT adsorbed with MB by cross-linking with glutaraldehyde. The dependence on the biosensor response for ethanol was investigated in terms of pH, supporting electrolyte, ADH and NAD(+) amounts and working potential. The amperometric response for alcohol using this biosensor showed excellent sensitivity (4.75 mu A cm(-2) mmol L-1), operational stability (around 95% of the activity was maintained after 300 determinations) and wide linear response range (0.05-10 mmol L-1)(.) These favorable characteristics allowed its application for measurements of ethanol in a great variety of alcoholic beverages with a simple dilution. The precision and recovery data showed by the proposed biosensor may give reliable results for real complex matrices. (c) 2006 Elsevier Ltd. All rights reserved.
dc.description52
dc.description1
dc.description215
dc.description220
dc.languageen
dc.publisherPergamon-elsevier Science Ltd
dc.publisherOxford
dc.publisherInglaterra
dc.relationElectrochimica Acta
dc.relationElectrochim. Acta
dc.rightsfechado
dc.rightshttp://www.elsevier.com/about/open-access/open-access-policies/article-posting-policy
dc.sourceWeb of Science
dc.subjectcarbon nanotube paste electrode
dc.subjectenzyme biosensor
dc.subjectalcohol dehydrogenase
dc.subjectMeldola's Blue
dc.subjectethanol determination
dc.subjectModified Electrodes
dc.subjectNiobium Oxide
dc.subjectElectrochemical Biosensors
dc.subjectPaste Electrodes
dc.subjectMethylene-blue
dc.subjectEnzymes
dc.subjectSensor
dc.subjectEnds
dc.subjectOxidation
dc.subjectGraphite
dc.titleAmperometric biosensor for ethanol based on co-immobilization of alcohol dehydrogenase and Meldola's Blue on multi-wall carbon nanotube
dc.typeArtículos de revistas


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