Chilling stress leads to increased cell membrane rigidity in roots of coffee (Coffea arabica L) seedlings

dc.creatorAlonso, A
dc.creatorQueiroz, CS
dc.creatorMagalhaes, AC
dc.date1997
dc.date41640
dc.date2014-12-16T11:35:02Z
dc.date2015-11-26T18:03:47Z
dc.date2014-12-16T11:35:02Z
dc.date2015-11-26T18:03:47Z
dc.date.accessioned2018-03-29T00:45:46Z
dc.date.available2018-03-29T00:45:46Z
dc.identifierBiochimica Et Biophysica Acta-biomembranes. Elsevier Science Bv, v. 1323, n. 1, n. 75, n. 84, 1997.
dc.identifier0005-2736
dc.identifierWOS:A1997WD78000009
dc.identifier10.1016/S0005-2736(96)00177-0
dc.identifierhttp://www.repositorio.unicamp.br/jspui/handle/REPOSIP/63100
dc.identifierhttp://www.repositorio.unicamp.br/handle/REPOSIP/63100
dc.identifierhttp://repositorio.unicamp.br/jspui/handle/REPOSIP/63100
dc.identifier.urihttp://repositorioslatinoamericanos.uchile.cl/handle/2250/1292654
dc.descriptionTropical and sub-tropical higher plant species show marked growth inhibition when exposed to chilling temperatures. In root tip segments of coffee seedlings which were subjected for 6 days to temperatures of 10, 15, 20 and 25 degrees C, in darkness, we have detected an increased amount of malondialdehyde formed in the 10 degrees C treatment, accompanied by higher electrolyte leakage. The electron paramagnetic resonance (EPR) technique and the fatty acid spin probes 5-, 12- and 16-doxylstearic acid were used to assess cellular membrane fluidity. At the depth of the 5th and 16th carbon atom of the alkyI chains the nitroxide radical detected more rigid membranes in seedlings subjected to 10 degrees C compared with 15 and 25 degrees C. At the C-12 position of the chains the probe showed very restricted motion and was insensitive to chilling induced membrane alterations. EPR parameters for intact tissues and microsome preparations from root tips showed that the fluidity was essentially the same when evaluated at C-5 and C-16 positions of the chains, and was considerably more fluid for microsomal membranes in the region of the C-12 position of the bilayers. The rotational motion of the nitroxide at C-16 position of the chains experienced a phase transition at about 15 degrees C. The calculated energy barriers for reorientational motion of the probe 16-doxylstearic acid were higher at temperatures of 5-15 degrees C than in the interval of 15-25 degrees C, suggesting that below the phase transition the membrane lipids assume a more ordered and compacted array. Membrane rigidity induced by chilling was interpreted as due to lipid peroxidation that could have been facilitated by higher density of peroxidizable chains below the membrane phase transition.
dc.description1323
dc.description1
dc.description75
dc.description84
dc.languageen
dc.publisherElsevier Science Bv
dc.publisherAmsterdam
dc.publisherHolanda
dc.relationBiochimica Et Biophysica Acta-biomembranes
dc.relationBiochim. Biophys. Acta-Biomembr.
dc.rightsfechado
dc.rightshttp://www.elsevier.com/about/open-access/open-access-policies/article-posting-policy
dc.sourceWeb of Science
dc.subjectchilling stress
dc.subjectmembrane fluidity
dc.subjectlipid peroxidation
dc.subjectEPR
dc.subject(Coffea arabica L)
dc.subjectMicrosomal Lipid-peroxidation
dc.subjectElectron-spin Resonance
dc.subjectSuperoxide-dismutase
dc.subjectInjury
dc.subjectLeaves
dc.subjectPermeability
dc.subjectSensitivity
dc.subjectTemperature
dc.subjectResistant
dc.subjectFluidity
dc.titleChilling stress leads to increased cell membrane rigidity in roots of coffee (Coffea arabica L) seedlings
dc.typeArtículos de revistas


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