Amplification and protein expression of chromosome 12q13-15 genes in osteosarcomas of the jaws

dc.creatorLopes, MA
dc.creatorNikitakis, NG
dc.creatorOrd, RA
dc.creatorSauk, J
dc.date2001
dc.dateOCT
dc.date2014-11-19T14:00:25Z
dc.date2015-11-26T18:03:40Z
dc.date2014-11-19T14:00:25Z
dc.date2015-11-26T18:03:40Z
dc.date.accessioned2018-03-29T00:45:37Z
dc.date.available2018-03-29T00:45:37Z
dc.identifierOral Oncology. Pergamon-elsevier Science Ltd, v. 37, n. 7, n. 566, n. 571, 2001.
dc.identifier0964-1955
dc.identifierWOS:000170933200005
dc.identifier10.1016/S1368-8375(00)00130-5
dc.identifierhttp://www.repositorio.unicamp.br/jspui/handle/REPOSIP/54260
dc.identifierhttp://www.repositorio.unicamp.br/handle/REPOSIP/54260
dc.identifierhttp://repositorio.unicamp.br/jspui/handle/REPOSIP/54260
dc.identifier.urihttp://repositorioslatinoamericanos.uchile.cl/handle/2250/1292612
dc.descriptionOverexpression and amplification of several genes (MDM2, CDK4 and SAS) located on chromosome 12q13-15 have been noted to occur in various human sarcomas. As a result, two major growth regulation pathways may be inhibited. MDM2 may down regulate the p53-mediated growth control and CDK4 may affect pRB-mediated events. To determine the frequency of alterations in these genes and their correlation with clinicopathologic features, we analyzed the MDM2 and CDK4 protein levels by immunohistochemistry and assessed MDM2, CDK4 and SAS amplification by real-time PCR in nine osteosarcomas of the jaws. Positive staining for CDK4 and MDM2 was observed in eight cases (88.8%) and five cases (55.5%), respectively. Intense CDK4 staining was noted in four cases (two high grade, one intermediate grade and one low grade). Intense MDM2 staining was observed in the same four previous cases, as well as, one additional high-grade tumor. Individual DNA amplification for CDK4, MDM2 and SAS was observed in six cases for each gene. Co-amplification was observed in five cases that showed CDK4 and MDM2 concomitant amplification and four cases that displayed amplification for all of the genes. In addition, among the five cases that presented CDK4 and MDM2 amplification, strong overexpression of CDK4 and MDM2 was observed in three and in four cases, respectively (three high grade and one intermediate grade). These results suggest that 12q13-15 genes are involved in neoplastic disease and concurrent amplification and overexpression of these genes might help to define high-grade tumors. (C) 2001 Elsevier Science Ltd. All rights reserved.
dc.description37
dc.description7
dc.description566
dc.description571
dc.languageen
dc.publisherPergamon-elsevier Science Ltd
dc.publisherOxford
dc.publisherInglaterra
dc.relationOral Oncology
dc.relationOral Oncol.
dc.rightsfechado
dc.rightshttp://www.elsevier.com/about/open-access/open-access-policies/article-posting-policy
dc.sourceWeb of Science
dc.subjectosteosarcoma
dc.subjectjaw
dc.subjectMDM2
dc.subjectCDK4
dc.subjectSAS
dc.subjectimmunohistochemistry
dc.subjectreal-time PCR
dc.subjectSoft-tissue Tumors
dc.subjectHuman Sarcomas
dc.subjectOsteogenic-sarcoma
dc.subjectMdm2 Amplification
dc.subjectP53 Mutations
dc.subjectCdk4 Gene
dc.subjectHead
dc.subjectNeck
dc.subjectSas
dc.subjectExperience
dc.titleAmplification and protein expression of chromosome 12q13-15 genes in osteosarcomas of the jaws
dc.typeArtículos de revistas


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