Quantitation and identity confirmation of residues of quinolones in tilapia fillets by LC-ESI-MS-MS QToF

dc.creatorPaschoal, JAR
dc.creatorReyes, FGR
dc.creatorRath, S
dc.date2009
dc.dateAUG
dc.date2014-11-19T12:29:15Z
dc.date2015-11-26T18:03:05Z
dc.date2014-11-19T12:29:15Z
dc.date2015-11-26T18:03:05Z
dc.date.accessioned2018-03-29T00:44:52Z
dc.date.available2018-03-29T00:44:52Z
dc.identifierAnalytical And Bioanalytical Chemistry. Springer Heidelberg, v. 394, n. 8, n. 2213, n. 2221, 2009.
dc.identifier1618-2642
dc.identifier1618-2650
dc.identifierWOS:000268478100026
dc.identifier10.1007/s00216-009-2900-z
dc.identifierhttp://www.repositorio.unicamp.br/jspui/handle/REPOSIP/58622
dc.identifierhttp://www.repositorio.unicamp.br/handle/REPOSIP/58622
dc.identifierhttp://repositorio.unicamp.br/jspui/handle/REPOSIP/58622
dc.identifier.urihttp://repositorioslatinoamericanos.uchile.cl/handle/2250/1292425
dc.descriptionConselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)
dc.descriptionFundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)
dc.descriptionA method for simultaneous determination of flumequine (FLM), oxolinic acid (OXO), sarafloxacin (SAR), danofloxacin (DAN), enrofloxacin (ENR), and ciprofloxacin (CIP) in tilapia (Orechromis niloticus) fillets, using liquid chromatography-tandem mass spectrometry (LC-ESI-MS-MS QToF) is presented. The quinolones were extracted from the food matrix with a solution of 10% trichloroacetic acid-methanol (80:20 v/v) with ultrasonic assistance. Clean-up of the extract solution was performed by using polymeric solid-phase extraction cartridges. The LC separation was carried out on an octadecyl hybrid silica column (C-18, 150 mm x 3 mm, 5 mu m). The column temperature was set at 30 A degrees C, and gradient elution (0.2 mL min(-1)) was performed using water and acetonitrile, both containing 0.1% of acetic acid, as mobile phase components. The analytes were ionized using electrospray in the positive polarity mode. The following analytical results were obtained: linearity was about 0.99 for all the quinolones; intra and inter-assay precision (RSD%) were lower than 12.7 and 20%, respectively; and recoveries were from 89 to 112%. The quantitation limits were below the maximum residue limits established for the analytes. The method is suitable for the determination of quinolone residues in fish fillets and the QToF technique made it possible to obtain m/z ratios with less than 10 ppm of error for each analyte.
dc.description394
dc.description8
dc.description2213
dc.description2221
dc.descriptionConselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)
dc.descriptionFundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)
dc.descriptionConselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)
dc.descriptionFundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)
dc.languageen
dc.publisherSpringer Heidelberg
dc.publisherHeidelberg
dc.publisherAlemanha
dc.relationAnalytical And Bioanalytical Chemistry
dc.relationAnal. Bioanal. Chem.
dc.rightsfechado
dc.rightshttp://www.springer.com/open+access/authors+rights?SGWID=0-176704-12-683201-0
dc.sourceWeb of Science
dc.subjectQuinolones
dc.subjectFish
dc.subjectTilapia
dc.subjectLC-MS-MS QToF
dc.subjectChromatography-mass-spectrometry
dc.subjectLiquid-chromatography
dc.subjectFluorescence Detection
dc.subjectMalachite Green
dc.subjectMuscle
dc.subjectValidation
dc.subjectTissues
dc.subjectTetracyclines
dc.subjectPesticides
dc.subjectGuidelines
dc.titleQuantitation and identity confirmation of residues of quinolones in tilapia fillets by LC-ESI-MS-MS QToF
dc.typeArtículos de revistas


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