Effect of a trypsin inhibitor from Dimorphandra mollis seeds on the development of Callosobruchus maculatus

dc.creatorMacedo, MLR
dc.creatorMello, GC
dc.creatorFreire, MDGM
dc.creatorNovello, JC
dc.creatorMarangoni, S
dc.creatorde Matos, DGG
dc.date2002
dc.dateOCT
dc.date2014-11-19T11:58:34Z
dc.date2015-11-26T18:02:51Z
dc.date2014-11-19T11:58:34Z
dc.date2015-11-26T18:02:51Z
dc.date.accessioned2018-03-29T00:44:37Z
dc.date.available2018-03-29T00:44:37Z
dc.identifierPlant Physiology And Biochemistry. Gauthier-villars/editions Elsevier, v. 40, n. 10, n. 891, n. 898, 2002.
dc.identifier0981-9428
dc.identifierWOS:000179205000011
dc.identifier10.1016/S0981-9428(02)01441-9
dc.identifierhttp://www.repositorio.unicamp.br/jspui/handle/REPOSIP/63716
dc.identifierhttp://www.repositorio.unicamp.br/handle/REPOSIP/63716
dc.identifierhttp://repositorio.unicamp.br/jspui/handle/REPOSIP/63716
dc.identifier.urihttp://repositorioslatinoamericanos.uchile.cl/handle/2250/1292356
dc.descriptionBruchid larvae cause major losses in grain legume crops throughout the world. Some bruchid species, such as the cowpea weevil, are pests that damage stored seeds. Plants synthesize a variety of molecules, including proteinaceous proteinase inhibitors, to defend themselves against attack by insects. In this work, a trypsin inhibitor (DMTI-II) isolated from Dimorphandra mollis seeds was tested for anti-insect activity against Callosobruchus maculatus larvae. The inhibitor produced ca. 67% mortality to this bruchid when incorporated into an artificial diet at a level of 1%. The doses necessary to cause 50% mortality (LD50) and to reduce weight by 50% (ED50) for DMTI-II were ca. 0.50% and 0.60%, respectively. The action of DMTI-II on C. maculatus larvae may involve the inhibition of trypsin-like activity of larval midgut extracts, the absence of digestion by midgut preparations or with a mixture of pepsin and papain, and its association with a chitin column and chitinous structure in the midgut of this insect. (C) 2002 Editions scientifiques et medicales Elsevier SAS. All rights reserved.
dc.description40
dc.description10
dc.description891
dc.description898
dc.languageen
dc.publisherGauthier-villars/editions Elsevier
dc.publisherParis
dc.publisherFrança
dc.relationPlant Physiology And Biochemistry
dc.relationPlant Physiol. Biochem.
dc.rightsfechado
dc.rightshttp://www.elsevier.com/about/open-access/open-access-policies/article-posting-policy
dc.sourceWeb of Science
dc.subjectCallosobruchus maculatus
dc.subjectdimorphandramollis
dc.subjectresistance
dc.subjecttrypsin inhibitor
dc.subjectVigna-unguiculata Seeds
dc.subjectPlant Protease Inhibitors
dc.subjectZabrotes-subfasciatus
dc.subjectProteinase-inhibitors
dc.subjectCysteine Proteinases
dc.subjectInsect-resistant
dc.subjectLarvae
dc.subjectPotato
dc.subjectMidgut
dc.subjectColeoptera
dc.titleEffect of a trypsin inhibitor from Dimorphandra mollis seeds on the development of Callosobruchus maculatus
dc.typeArtículos de revistas


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