Dermatan sulfate and bone marrow mononuclear cells used as a new therapeutic strategy after arterial injury in mice

dc.creatorGodoy, JAP
dc.creatorBlock, DB
dc.creatorTollefsen, DM
dc.creatorWerneck, CC
dc.creatorVicente, CP
dc.date2011
dc.dateJUL
dc.date2014-08-01T18:29:09Z
dc.date2015-11-26T18:01:55Z
dc.date2014-08-01T18:29:09Z
dc.date2015-11-26T18:01:55Z
dc.date.accessioned2018-03-29T00:43:33Z
dc.date.available2018-03-29T00:43:33Z
dc.identifierCytotherapy. Informa Healthcare, v. 13, n. 6, n. 695, n. 704, 2011.
dc.identifier1465-3249
dc.identifierWOS:000291658200006
dc.identifier10.3109/14653249.2010.548378
dc.identifierhttp://www.repositorio.unicamp.br/jspui/handle/REPOSIP/79522
dc.identifierhttp://repositorio.unicamp.br/jspui/handle/REPOSIP/79522
dc.identifier.urihttp://repositorioslatinoamericanos.uchile.cl/handle/2250/1292098
dc.descriptionFundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)
dc.descriptionConselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)
dc.descriptionCoordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)
dc.descriptionBackground aims. Previously, we have demonstrated that administration of dermatan sulfate (DS) suppresses neointima formation in the mouse carotid artery by activating heparin co-factor II. A similar suppressive effect was observed by increasing the number of progenitor cells in circulation. In this study, we investigated the combination of DS and bone marrow mononuclear cells (MNC), which includes potential endothelial progenitors, in neointima formation after arterial injury. Methods. Arterial injury was induced by mechanical dilation of the left common carotid artery. We analyzed the extension of endothelial lesion, thrombus formation, P-selectin expression and CD45(+) cell accumulation 1 and 3 days post-injury, and neointima formation 21 days post-injury. Animals were injected with MNC with or without DS during the first 48 h after injury. Results. The extension of endothelial lesion was similar in all groups 1 day after surgery; however, in injured animals treated with MNC and DS the endothelium recovery seemed to be more efficient 21 days after lesion. Treatment with DS inhibited thrombosis, decreased CD45(+) cell accumulation and P-selectin expression at the site of injury, and reduced the neointimal area by 56%. Treatment with MNC reduced the neointimal area by 54%. The combination of DS and MNC reduced neointima formation by more than 91%. In addition, DS promoted a greater accumulation of MNC at the site of injury. Conclusions. DS inhibits the initial thrombotic and inflammatory processes after arterial injury and promotes migration of MNC to the site of the lesion, where they may assist in the recovery of the injured endothelium.
dc.description13
dc.description6
dc.description695
dc.description704
dc.descriptionFundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)
dc.descriptionConselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)
dc.descriptionNHLBI [HL55520]
dc.descriptionCoordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)
dc.descriptionFundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)
dc.descriptionConselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)
dc.descriptionCoordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)
dc.descriptionFAPESP [2007/01112-6, 2009/00950-3, 2010/01119-3, 2010/11474-5]
dc.descriptionCNPq [472087/2008-8]
dc.descriptionNHLBI [HL55520]
dc.languageen
dc.publisherInforma Healthcare
dc.publisherLondon
dc.publisherInglaterra
dc.relationCytotherapy
dc.relationCytotherapy
dc.rightsfechado
dc.rightshttp://informahealthcare.com/userimages/ContentEditor/1255620309227/Copyright_And_Permissions.pdf
dc.sourceWeb of Science
dc.subjectbone marrow cells
dc.subjectdermatan sulfate
dc.subjectinflammation
dc.subjectrestenosis
dc.subjectthrombosis
dc.subjectEndothelial Progenitor Cells
dc.subjectHeparin-cofactor-ii
dc.subjectDeficient Mice
dc.subjectP-selectin
dc.subjectNeointima Formation
dc.subjectAtherosclerosis
dc.subjectThrombosis
dc.titleDermatan sulfate and bone marrow mononuclear cells used as a new therapeutic strategy after arterial injury in mice
dc.typeArtículos de revistas


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