Inhibition of the activity of matrix metalloproteinase 2 by triethylene glycol dimethacrylate

dc.creatorde Carvalho, RV
dc.creatorOgliari, FA
dc.creatorMarques, MR
dc.creatorde Souza, AP
dc.creatorPetzhold, CL
dc.creatorLine, SRP
dc.creatorPiva, E
dc.creatorEtges, A
dc.date2011
dc.dateOCT
dc.date2014-08-01T18:33:15Z
dc.date2015-11-26T18:01:31Z
dc.date2014-08-01T18:33:15Z
dc.date2015-11-26T18:01:31Z
dc.date.accessioned2018-03-29T00:43:07Z
dc.date.available2018-03-29T00:43:07Z
dc.identifierClinical Oral Investigations. Springer Heidelberg, v. 15, n. 5, n. 643, n. 648, 2011.
dc.identifier1432-6981
dc.identifierWOS:000296888600006
dc.identifier10.1007/s00784-010-0443-5
dc.identifierhttp://www.repositorio.unicamp.br/jspui/handle/REPOSIP/80640
dc.identifierhttp://repositorio.unicamp.br/jspui/handle/REPOSIP/80640
dc.identifier.urihttp://repositorioslatinoamericanos.uchile.cl/handle/2250/1291998
dc.descriptionCoordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)
dc.descriptionThe aim of this study was to evaluate the effect of different concentrations of triethylene glycol dimethacrylate (TEGDMA) on the inhibition of matrix metalloproteinase 2 (MMP-2). Mouse gingival explants were cultured overnight in DMEM and the expression of secreted enzymes was analyzed by gelatin zymography in buffers containing 5 mM CaCl(2) (Tris-CaCl(2)) in 50 mM Tris-HCl buffer with the addition of TEGDMA at different concentrations (0.62%, 1.25%, 2.5%, or 5.0% (v/v)). The gelatinolytic proteinase present in the conditioned media was characterized as matrix metalloproteinase by means of specific chemical inhibition. The matrix metalloproteinases present in the conditioned media were characterized as MMP-2 by immunoprecipitation. The eletrophoretic bands were scanned and the transmittance values were analyzed. Data was plotted and submitted to linear regression to investigate MMP-2 inhibition as a function of TEGDMA concentration. Three major bands were detected in the zymographic assays. These bands were characterized as MMP-2. Zymogene (72 kDa), intermediate (66 kDa) and active forms of MMP-2 (62 kDa) were inhibited by TEGDMA in a dose-dependent way. These findings suggest that TEGDMA could inhibit MMP-2 expression even at small concentrations.
dc.description15
dc.description5
dc.description643
dc.description648
dc.descriptionCoordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)
dc.descriptionAngelus Science and Technology
dc.descriptionCoordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)
dc.languageen
dc.publisherSpringer Heidelberg
dc.publisherHeidelberg
dc.publisherAlemanha
dc.relationClinical Oral Investigations
dc.relationClin. Oral Investig.
dc.rightsfechado
dc.rightshttp://www.springer.com/open+access/authors+rights?SGWID=0-176704-12-683201-0
dc.sourceWeb of Science
dc.subjectGelatinase A
dc.subjectMatrix metalloproteinase 2
dc.subjectTriethyleneglycol dimethacrylate
dc.subjectTEGDMA
dc.subjectPolyacrylamide gel electrophoresis
dc.subjectSelf-etching Adhesives
dc.subjectLayers In-vivo
dc.subjectGelatinases Mmp-2
dc.subjectTransition-metal
dc.subjectCrown-ethers
dc.subjectDentin
dc.subjectChlorhexidine
dc.subjectDegradation
dc.subjectTegdma
dc.subjectActivation
dc.titleInhibition of the activity of matrix metalloproteinase 2 by triethylene glycol dimethacrylate
dc.typeArtículos de revistas


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