The single cell protein and extracellular enzyme production from a lignocellulolytic fungus, Chrysonilia sitophila, using different carbon sources were evaluated. The mycelial dry mass composition showed a high protein (39.2%) and low nucleic acid content (3.3%), as well as carbohydrate, fatty acid, fiber, and ash levels comparable with single cell proteins currently studied. Mycelial protein showed amino acid content similar to or higher than FAO standard requirements. The amino acid, fatty acid, and carotenoid composition, as well as mycelial mass yield and enzyme production, were dependent on the carbon source used. Glucose, saccharose, cellobiose, cellulose, microcrystalline cellulose, lactose, and rice hull as carbon sources were studied. The major mycelial dry mass yield was 0.38 g of fungi/g of total substrate with 1.0% glucose as the carbon source in 6-d batch cultures. The maximal extracellular enzyme production was 40 U/L filter paper activity, 20 U/L beta-glucosidase, 200 U/L endoglucanase, and 190 U/L protease.273267276