Cell-to-cell contact dependence and junctional protein content are correlated with in vivo maturation of pancreatic beta cells

dc.creatorSantos-Silva, JC
dc.creatorCarvalho, CPD
dc.creatorde Oliveira, RB
dc.creatorBoschero, AC
dc.creatorCollares-Buzato, CB
dc.date2012
dc.dateJUL
dc.date2014-08-01T18:24:56Z
dc.date2015-11-26T18:01:01Z
dc.date2014-08-01T18:24:56Z
dc.date2015-11-26T18:01:01Z
dc.date.accessioned2018-03-29T00:42:33Z
dc.date.available2018-03-29T00:42:33Z
dc.identifierCanadian Journal Of Physiology And Pharmacology. Canadian Science Publishing, Nrc Research Press, v. 90, n. 7, n. 837, n. 850, 2012.
dc.identifier0008-4212
dc.identifierWOS:000306110100003
dc.identifier10.1139/Y2012-064
dc.identifierhttp://www.repositorio.unicamp.br/jspui/handle/REPOSIP/78587
dc.identifierhttp://repositorio.unicamp.br/jspui/handle/REPOSIP/78587
dc.identifier.urihttp://repositorioslatinoamericanos.uchile.cl/handle/2250/1291870
dc.descriptionCoordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)
dc.descriptionFundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)
dc.descriptionIn this study, we investigated the cellular distribution of junctional proteins and the dependence on cell-cell contacts of pancreatic beta cells during animal development. Fetus and newborn rat islets, which display a relatively poor insulin secretory response to glucose, present an immature morphology and cytoarchitecture when compared with young and adult islets that are responsive to glucose. At the perinatal stage, beta cells display a low junctional content of neural cell adhesion molecule (N-CAM), alpha-and beta-catenins, ZO-1, and F-actin, while a differential distribution of N-CAM and Pan-cadherin was seen in beta cells and nonbeta cells only from young and adult islets. In the absence of intercellular contacts, the glucose-stimulated insulin secretion was completely blocked in adult beta cells, but after reaggregation they partially re-established the secretory response to glucose. By contrast, neonatal beta cells were poorly responsive to sugar, regardless of whether they were arranged as intact islets or as isolated cells. Interestingly, after 10 days of culturing, neonatal beta cells, known to display increased junctional protein content in vitro, became responsive to glucose and concomitantly dependent on cell-cell contacts. Therefore, our data suggest that the developmental acquisition of an adult-like insulin secretory pattern is paralleled by a dependence on direct cell-cell interactions.
dc.description90
dc.description7
dc.description837
dc.description850
dc.descriptionCoordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)
dc.descriptionFundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)
dc.descriptionCoordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)
dc.descriptionFundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)
dc.descriptionFAPESP [10/50789-1]
dc.languageen
dc.publisherCanadian Science Publishing, Nrc Research Press
dc.publisherOttawa
dc.publisherCanadá
dc.relationCanadian Journal Of Physiology And Pharmacology
dc.relationCan. J. Physiol. Pharmacol.
dc.rightsfechado
dc.sourceWeb of Science
dc.subjectpancreatic beta cells
dc.subjectintercellular junctions
dc.subjectbeta cell maturation
dc.subjectpancreas development
dc.subjectcell adhesion
dc.subjectjunctional proteins
dc.subjectinsulin secretion
dc.subjectInsulin Secretory Responses
dc.subjectNeonatal-rat Islets
dc.subjectMolecule N-cam
dc.subjectE-cadherin
dc.subjectAdherens Junction
dc.subjectAdhesion Molecule
dc.subjectB-cells
dc.subjectIntercellular Communication
dc.subjectEndocrine Pancreas
dc.subjectCardiac Myocytes
dc.titleCell-to-cell contact dependence and junctional protein content are correlated with in vivo maturation of pancreatic beta cells
dc.typeArtículos de revistas


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