Agelaia MP-I: A peptide isolated from the venom of the social wasp, Agelaia pallipes pallipes, enhances insulin secretion in mice pancreatic islets

dc.creatorBaptista-Saidemberg, NB
dc.creatorSaidemberg, DM
dc.creatorRibeiro, RA
dc.creatorArcuri, HA
dc.creatorPalma, MS
dc.creatorCarneiro, EM
dc.date2012
dc.dateSEP 15
dc.date2014-08-01T18:18:36Z
dc.date2015-11-26T17:59:48Z
dc.date2014-08-01T18:18:36Z
dc.date2015-11-26T17:59:48Z
dc.date.accessioned2018-03-29T00:42:08Z
dc.date.available2018-03-29T00:42:08Z
dc.identifierToxicon. Pergamon-elsevier Science Ltd, v. 60, n. 4, n. 596, n. 602, 2012.
dc.identifier0041-0101
dc.identifierWOS:000306777100017
dc.identifier10.1016/j.toxicon.2012.05.027
dc.identifierhttp://www.repositorio.unicamp.br/jspui/handle/REPOSIP/76975
dc.identifierhttp://repositorio.unicamp.br/jspui/handle/REPOSIP/76975
dc.identifier.urihttp://repositorioslatinoamericanos.uchile.cl/handle/2250/1291766
dc.descriptionFundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)
dc.descriptionCoordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)
dc.descriptionPeptides isolated from animal venoms have shown the ability to regulate pancreatic beta cell function. Characterization of wasp venoms is important, since some components of these venoms present large molecular variability, and potential interactions with different signal transduction pathways. For example, the well studied mastoparan peptides interact with a diversity of cell types and cellular components and stimulate insulin secretion via the inhibition of ATP dependent K+ (K-ATP) channels, increasing intracellular Ca2+ concentration. In this study, the insulin secretion of isolated pancreatic islets from adult Swiss mice was evaluated in the presence of synthetic Agelaia MP-I (AMP-I) peptide, and some mechanisms of action of this peptide on endocrine pancreatic function were characterized. AMP-I was manually synthesized using the Fmoc strategy, purified by RP-HPLC and analyzed using ESI-IT-TOF mass spectrometry. Isolated islets were incubated at increasing glucose concentrations (2.8, 11.1 and 22.2 mM) without (Control group: CTL) or with 10 mu M AMP-I (AMP-I group). AMP-I increased insulin release at all tested glucose concentrations, when compared with CTL (P < 0.05). Since molecular analysis showed a potential role of the peptide interaction with ionic channels, insulin secretion was also analyzed in the presence of 250 mu M diazoxide, a K-ATP channel opener and 10 mu M nifedipine, a Ca2+ channel blocker. These drugs abolished insulin secretion in the CTL group in the presence of 2.8 and 11.1 mM glucose, whereas. AMP-I also enhanced insulin secretory capacity, under these glucose conditions, when incubated with diazoxide and nifedipine. In conclusion, AMP-I increased beta cell secretion without interfering in K-ATP and L-type Ca2+ channel function, suggesting a different mechanism for this peptide, possibly by G protein interaction, due to the structural similarity of this peptide with Mastoparan-X, as obtained by modeling. (c) 2012 Elsevier Ltd. All rights reserved.
dc.description60
dc.description4
dc.descriptionSI
dc.description596
dc.description602
dc.descriptionFundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)
dc.descriptionCoordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)
dc.descriptionFundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)
dc.descriptionCoordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)
dc.descriptionFAPESP [2011/51684-1]
dc.languageen
dc.publisherPergamon-elsevier Science Ltd
dc.publisherOxford
dc.publisherInglaterra
dc.relationToxicon
dc.relationToxicon
dc.rightsfechado
dc.rightshttp://www.elsevier.com/about/open-access/open-access-policies/article-posting-policy
dc.sourceWeb of Science
dc.subjectMastoparan
dc.subjectWasp venom
dc.subjectSynthetic peptides
dc.subjectInsulin secretion
dc.subjectProtein-bound Conformation
dc.subjectArachidonic-acid Release
dc.subjectPeritoneal Mast-cells
dc.subjectMastoparan-x
dc.subjectPhosphoinositide Hydrolysis
dc.subjectAmphiphilic Peptides
dc.subjectCalcium Influx
dc.subjectSubstance-p
dc.subjectBeta-cell
dc.subjectRat
dc.titleAgelaia MP-I: A peptide isolated from the venom of the social wasp, Agelaia pallipes pallipes, enhances insulin secretion in mice pancreatic islets
dc.typeArtículos de revistas


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