FGF2 alters expression of the pyrophosphate/phosphate regulating proteins, PC-1, ANK and TNAP, in the calvarial osteoblastic cell line, MC3T3E1 (C4)

dc.creatorHatch, NE
dc.creatorNociti, F
dc.creatorSwanson, E
dc.creatorBothwell, M
dc.creatorSomerman, M
dc.date2005
dc.date2014-11-19T06:31:45Z
dc.date2015-11-26T17:58:19Z
dc.date2014-11-19T06:31:45Z
dc.date2015-11-26T17:58:19Z
dc.date.accessioned2018-03-29T00:41:52Z
dc.date.available2018-03-29T00:41:52Z
dc.identifierConnective Tissue Research. Taylor & Francis Ltd, v. 46, n. 41763, n. 184, n. 192, 2005.
dc.identifier0300-8207
dc.identifierWOS:000235434000005
dc.identifier10.1080/03008200500237203
dc.identifierhttp://www.repositorio.unicamp.br/jspui/handle/REPOSIP/66900
dc.identifierhttp://www.repositorio.unicamp.br/handle/REPOSIP/66900
dc.identifierhttp://repositorio.unicamp.br/jspui/handle/REPOSIP/66900
dc.identifier.urihttp://repositorioslatinoamericanos.uchile.cl/handle/2250/1291701
dc.descriptionFibroblast growth factor/fibroblast growth factor receptor (FGF/FGFR) signaling has been linked to the aberrant mineralization phenotype of craniosynostosis syndromes. One critical aspect of mineralization involves the elaboration and transport of pyrophosphate into the extracellular matrix with subsequent enzymatic hydrolysis into phosphate. Altered expression of the pyrophosphate elaborating factors, TNAP (tissue nonspecific alkaline phosphatase), PC-1, and ANK, downstream of FGF/FGFR signaling may provide a potential mechanism for the craniosynostosis phenotype. As an initial step toward testing this hypothesis, we confirmed that ANK mRNA is upregulated during osteoblast differentiation in culture. Subsequently, the effect of FGF2 treatment on expression of PC-1, ANK, and TNAP in the calvarial osteoblastic cell line, MC3T3E1(C4), was investigated. FGF2 specifically induced expression of PC-1 and ANK while inhibiting expression of TNAP, at both mRNA and protein levels. Concordant with these changes in gene expression, FGF2 inhibited mineralization. These results suggest that FGF/FGFR signaling may affect mineralization via changes in the elaboration and metabolism of pyrophosphate.
dc.description46
dc.description41763
dc.description184
dc.description192
dc.languageen
dc.publisherTaylor & Francis Ltd
dc.publisherAbingdon
dc.publisherInglaterra
dc.relationConnective Tissue Research
dc.relationConnect. Tissue Res.
dc.rightsfechado
dc.rightshttp://journalauthors.tandf.co.uk/permissions/reusingOwnWork.asp
dc.sourceWeb of Science
dc.subjectbone mineralization
dc.subjectgrowth factors
dc.subjectmolecular pathways
dc.subjectosteoblasts
dc.subjectpyrophosphate
dc.subjectBone Morphogenetic Proteins
dc.subjectFibroblast Growth Factor-2
dc.subjectAlkaline-phosphatase
dc.subjectMembrane Glycoprotein-1
dc.subjectInorganic Pyrophosphate
dc.subjectKinase-c
dc.subjectCraniometaphyseal Dysplasia
dc.subjectSignaling Pathways
dc.subjectExtracellular Ppi
dc.subjectGene-expression
dc.titleFGF2 alters expression of the pyrophosphate/phosphate regulating proteins, PC-1, ANK and TNAP, in the calvarial osteoblastic cell line, MC3T3E1 (C4)
dc.typeArtículos de revistas


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