A quick procedure for the isolation of dimeric piratoxins-I and II, two myotoxins from Bothrops pirajai snake venom. N-terminal sequencing

dc.creatorToyama, MH
dc.creatorMancuso, LC
dc.creatorGiglio, JR
dc.creatorNovello, JC
dc.creatorOliveira, B
dc.creatorMarangoni, S
dc.date1995
dc.dateDEC
dc.date2014-07-30T13:42:40Z
dc.date2015-11-26T17:57:46Z
dc.date2014-07-30T13:42:40Z
dc.date2015-11-26T17:57:46Z
dc.date.accessioned2018-03-29T00:41:20Z
dc.date.available2018-03-29T00:41:20Z
dc.identifierBiochemistry And Molecular Biology International. Academic Press Aust, v. 37, n. 6, n. 1047, n. 1055, 1995.
dc.identifier1039-9712
dc.identifierWOS:A1995VG87700003
dc.identifierhttp://www.repositorio.unicamp.br/jspui/handle/REPOSIP/53656
dc.identifierhttp://repositorio.unicamp.br/jspui/handle/REPOSIP/53656
dc.identifier.urihttp://repositorioslatinoamericanos.uchile.cl/handle/2250/1291574
dc.descriptionTwo myotoxins, MP-I and MP-II, from Bothrops pirajai snake venom, have been purified by a quick high performance liquid chromatography (HPLC) procedure. Based on the HPLC coelution profile, amino acid composition, N-terminal sequence, polyacrylamide gel electrophoresis (PAGE) migration, as well as lack of phospholipase-A(2) (PLA(2)) and proteolytic activities, MP-I and MP-II were identified as piratoxin-I (PrTX-I) and II (PrTX-II), respectively. This procedure affords, aside the reduced operation time, a high yield (35% of the applied sample in terms of A(280nm)) Of MP-I, which is the major myotoxin of the venom. The N-terminal sequences of MP-I, MP-II, PrTX-I and PrTX-II, up to the 51st, 31st, 46th and 39th residues, respectively, have been determined, revealing MP-I (and hence PrTX-I) as a Lys-49 PLA(2)-like myotoxin. Both MP-I and MP-II have been shown, by SDS-PAGE, to occur in dimeric isoforms.
dc.description37
dc.description6
dc.description1047
dc.description1055
dc.languageen
dc.publisherAcademic Press Aust
dc.publisherMarrickville
dc.publisherAustralia
dc.relationBiochemistry And Molecular Biology International
dc.relationBiochem. Mol. Biol. Int.
dc.rightsfechado
dc.sourceWeb of Science
dc.subjectInsularis Jararaca-ilhoa
dc.subjectAmino-acid-sequence
dc.subjectPancreatic Phospholipase-a2
dc.subjectBiological-activity
dc.subjectFractionation
dc.titleA quick procedure for the isolation of dimeric piratoxins-I and II, two myotoxins from Bothrops pirajai snake venom. N-terminal sequencing
dc.typeArtículos de revistas


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