Reference Genes for High-Throughput Quantitative Reverse Transcription-PCR Analysis of Gene Expression in Organs and Tissues of Eucalyptus Grown in Various Environmental Conditions

dc.creatorCassan-Wang, H
dc.creatorSoler, M
dc.creatorYu, H
dc.creatorCamargo, ELO
dc.creatorCarocha, V
dc.creatorLadouce, N
dc.creatorSavelli, B
dc.creatorPaiva, JAP
dc.creatorLeple, JC
dc.creatorGrima-Pettenati, J
dc.date2012
dc.dateDEC
dc.date2014-08-01T18:32:09Z
dc.date2015-11-26T17:57:10Z
dc.date2014-08-01T18:32:09Z
dc.date2015-11-26T17:57:10Z
dc.date.accessioned2018-03-29T00:40:43Z
dc.date.available2018-03-29T00:40:43Z
dc.identifierPlant And Cell Physiology. Oxford Univ Press, v. 53, n. 12, n. 2101, n. 2116, 2012.
dc.identifier0032-0781
dc.identifierWOS:000312105400012
dc.identifier10.1093/pcp/pcs152
dc.identifierhttp://www.repositorio.unicamp.br/jspui/handle/REPOSIP/80362
dc.identifierhttp://repositorio.unicamp.br/jspui/handle/REPOSIP/80362
dc.identifier.urihttp://repositorioslatinoamericanos.uchile.cl/handle/2250/1291411
dc.descriptionFundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)
dc.descriptionInterest in the genomics of Eucalyptus has skyrocketed thanks to the recent sequencing of the genome of Eucalyptus grandis and to a growing number of large-scale transcriptomic studies. Quantitative reverse transcription-PCR (RT-PCR) is the method of choice for gene expression analysis and can now also be used as a high-throughput method. The selection of appropriate internal controls is becoming of utmost importance to ensure accurate expression results in Eucalyptus. To this end, we selected 21 candidate reference genes and used high-throughput microfluidic dynamic arrays to assess their expression among a large panel of developmental and environmental conditions with a special focus on wood-forming tissues. We analyzed the expression stability of these genes by using three distinct statistical algorithms (geNorm, NormFinder and delta Ct), and used principal component analysis to compare methods and rankings. We showed that the most stable genes identified depended not only on the panel of biological samples considered but also on the statistical method used. We then developed a comprehensive integration of the rankings generated by the three methods and identified the optimal reference genes for 17 distinct experimental sets covering 13 organs and tissues, as well as various developmental and environmental conditions. The expression patterns of Eucalyptus master genes EgMYB1 and EgMYB2 experimentally validated our selection. Our findings provide an important resource for the selection of appropriate reference genes for accurate and reliable normalization of gene expression data in the organs and tissues of Eucalyptus trees grown in a range of conditions including abiotic stresses.
dc.description53
dc.description12
dc.description2101
dc.description2116
dc.descriptionAgence Nationale pour la Recherche (ANR) [ANR-2010-KBBE-007-01]
dc.descriptionCentre National pour la Recherche Scientifique (CNRS)
dc.descriptionUniversity Paul Sabatier Toulouse III (UPS)
dc.descriptionFundacao para a Ciencia e Tecnologia (FCT) [P-KBBE/AGR_GPL/0001/2010, PTDC/AGR-GPL/098179/2008, PEst-OE/EQB/LA0004/2011]
dc.descriptionINTEREG IVB SudoE project Interbio
dc.descriptionLaboratoire d'Excellence (LABEX) project entitled TULIP [ANR-10-LABX-41]
dc.descriptionChina Scholarship Council
dc.descriptionFundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)
dc.descriptionFCT [SFRH/BD/72982/2010]
dc.descriptionDepartament d'Universitats, Recerca i Societat de la Informacio de la Generalitat de Catalunya
dc.descriptionFundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)
dc.descriptionAgence Nationale pour la Recherche (ANR) [ANR-2010-KBBE-007-01]
dc.descriptionFundacao para a Ciencia e Tecnologia (FCT) [P-KBBE/AGR_GPL/0001/2010, PTDC/AGR-GPL/098179/2008, PEst-OE/EQB/LA0004/2011]
dc.descriptionLaboratoire d'Excellence (LABEX) project entitled TULIP [ANR-10-LABX-41]
dc.descriptionFAPESP [FAPESP]
dc.descriptionFCT [SFRH/BD/72982/2010]
dc.languageen
dc.publisherOxford Univ Press
dc.publisherOxford
dc.publisherInglaterra
dc.relationPlant And Cell Physiology
dc.relationPlant Cell Physiol.
dc.rightsfechado
dc.rightshttp://www.oxfordjournals.org/access_purchase/self-archiving_policyb.html
dc.sourceWeb of Science
dc.subjectAbiotic stress
dc.subjectEucalyptus
dc.subjectGene expression
dc.subjectNormalization
dc.subjectReference genes
dc.subjectXylem
dc.subjectReal-time Pcr
dc.subjectCell-wall Formation
dc.subjectRt-pcr
dc.subjectWood Formation
dc.subjectSystematic Validation
dc.subjectLignin Biosynthesis
dc.subjectNormalization
dc.subjectIdentification
dc.subjectSelection
dc.subjectXylem
dc.titleReference Genes for High-Throughput Quantitative Reverse Transcription-PCR Analysis of Gene Expression in Organs and Tissues of Eucalyptus Grown in Various Environmental Conditions
dc.typeArtículos de revistas


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