Increased intraabdominal adipose tissue mass in fructose fed rats: Correction by metformin

dc.creatorBaret, G
dc.creatorPeyronnet, J
dc.creatorGrassi-Kassisse, D
dc.creatorDalmaz, Y
dc.creatorWiernsperger, N
dc.creatorGeloen, A
dc.date2002
dc.dateSEP
dc.date2014-11-19T01:55:05Z
dc.date2015-11-26T17:56:02Z
dc.date2014-11-19T01:55:05Z
dc.date2015-11-26T17:56:02Z
dc.date.accessioned2018-03-29T00:39:44Z
dc.date.available2018-03-29T00:39:44Z
dc.identifierExperimental And Clinical Endocrinology & Diabetes. Johann Ambrosius Barth Verlag Medizinverlage Heidelberg Gmbh, v. 110, n. 6, n. 298, n. 303, 2002.
dc.identifier0947-7349
dc.identifierWOS:000178698900008
dc.identifier10.1055/s-2002-34593
dc.identifierhttp://www.repositorio.unicamp.br/jspui/handle/REPOSIP/60020
dc.identifierhttp://www.repositorio.unicamp.br/handle/REPOSIP/60020
dc.identifierhttp://repositorio.unicamp.br/jspui/handle/REPOSIP/60020
dc.identifier.urihttp://repositorioslatinoamericanos.uchile.cl/handle/2250/1291166
dc.descriptionThe aim of the present study was to investigate the effect of metformin on insulin sensitivity, adipose tissue mass and sympathetic nervous system (SNS) activity in fructose fed rats. Male Sprague-Dawley rats were fed for six weeks either on a standard diet (C group) or on a high-fructose diet (F group, 10% in drinking water). In each group, half of the animals received metformin in drinking water for the last 4 weeks (500 mg/kg.day, C+M and F+M). Hyperinsulinemic-euglycemic clamps (6 mU insulin/kg.min) were performed on awake unrestrained rats to test insulin resistance. Six-week fructose diet induced a reproducible insulin resistance (31.1 +/- 1.9 C vs 22.5 +/- 3.2 mg glucose/kg.min F, p<0.05). Metformin treatment prevented insulin resistance (31.1 +/- 1.9 C vs 30.2 +/- 1.8 mg glucose/kg.min F+M, ns). To measure SNS activity, rats received, ten minutes before sacrifice, an i.p. injection of NSD (m-hydroxybenzylhydrazine, inhibitor of DOPA decarboxylase, 100 mg/kg). DOPA accumulation was used as an index of SNS activity and measured in superior cervical, coeliac ganglias, retroperitoneal and epidydimal adipose tissues. SNS activity was increased in F group only in coeliac ganglia (16.8 +/- 1.1 C vs 22.6 +/- 2.2 ng DOPA/ganglia, F group, p<0.05) and not in superior cervical ganglia (8.4 +/- 0.7 C vs 8.6 +/- 0.7 ng DOPA/ganglia, F group, ns). Metformin had no effect on SNS activity in coeliac ganglia of control animals (15.9 +/- 1.7 C+M vs 16.8 +/- 1.1 ng DOPA/coeliac ganglia C, ns) but prevented the increase in SNS activity in fructose fed animals (22.6 +/- 2.2 F vs 16.3 +/- 2.8 ng DOPA/coeliac ganglia F+M). In fructose fed rats, metformin significantly increased sympathetic activity in retroperitoneal white adipose tissue (RPWAT) resulting in a marked decrease in depot mass but had no effect on epidydimal WAT. In conclusion, our results demonstrate that fructose diet caused a selective increase of SNS activity in coeliac ganglia. Metformin increased SNS activity in RPWAT resulting in a significant reduction in RPWAT mass, lowered SNS activity in coeliac ganglia to control values and restore whole body insulin sensitivity.
dc.descriptiono TEXTO COMPLETO DESTE ARTIGO, ESTARÁ DISPONÍVEL À PARTIR DE AGOSTO DE 2015.
dc.description110
dc.description6
dc.description298
dc.description303
dc.languageen
dc.publisherJohann Ambrosius Barth Verlag Medizinverlage Heidelberg Gmbh
dc.publisherStuttgart
dc.publisherAlemanha
dc.relationExperimental And Clinical Endocrinology & Diabetes
dc.relationExp. Clin. Endocrinol. Diabet.
dc.rightsembargo
dc.sourceWeb of Science
dc.subjectinsulin resistance
dc.subjectfructose
dc.subjecthyperinsulinemic-euglycemic clamp
dc.subjectsympathetic nervous system
dc.subjectadipose tissue
dc.subjectInsulin-resistance
dc.subjectSecretion
dc.subjectGlucose
dc.titleIncreased intraabdominal adipose tissue mass in fructose fed rats: Correction by metformin
dc.typeArtículos de revistas


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