Increased expression of SNARE proteins and synaptotagmin IV in islets from pregnant rats and in vitro prolactin-treated neonatal islets

dc.creatorCunha, DA
dc.creatorAmaral, MEC
dc.creatorCarvalho, CPF
dc.creatorCollares-Buzato, CB
dc.creatorCarneiro, EM
dc.creatorBoschero, AC
dc.date2006
dc.date2014-11-19T01:51:10Z
dc.date2015-11-26T17:55:57Z
dc.date2014-11-19T01:51:10Z
dc.date2015-11-26T17:55:57Z
dc.date.accessioned2018-03-29T00:39:38Z
dc.date.available2018-03-29T00:39:38Z
dc.identifierBiological Research. Sociedad Biolgia Chile, v. 39, n. 3, n. 555, n. 566, 2006.
dc.identifier0716-9760
dc.identifierWOS:000241226100016
dc.identifierhttp://www.repositorio.unicamp.br/jspui/handle/REPOSIP/60017
dc.identifierhttp://www.repositorio.unicamp.br/handle/REPOSIP/60017
dc.identifierhttp://repositorio.unicamp.br/jspui/handle/REPOSIP/60017
dc.identifier.urihttp://repositorioslatinoamericanos.uchile.cl/handle/2250/1291140
dc.descriptionDuring pregnancy and the perinatal period of life, prolactin (PRL) and other lactogenic substances induce adaptation and maturation of the stimulus-secretion coupling system in pancreatic beta-cells. Since the SNARE molecules, SNAP-25, syntaxin 1, VAMP-2, and synaptotagmins participate in insulin secretion, we investigated whether the improved secretory response to glucose during these periods involves alteration in the expression of these proteins. mRNA was extracted from neonatal rat islets cultured for 5 days in the presence of PRL and from pregnant rats (17(th)-18(th) days of pregnancy) and reverse transcribed. The expression of genes was analyzed by semi-quantitative RT-PCR assay. The expression of proteins was analyzed by Western blotting and confocal microscopy. Transcription and expression of all SNARE genes and proteins were increased in islets from pregnant and PRL-treated neonatal rats when compared with controls. The only exception was VAMP-2 production in islets from pregnant rats. Increased mRNA and protein expression of synaptotagmin IV, but not the isoform I, also was observed in islets from pregnant and PRL-treated rats. This effect was not inhibited by wortmannin or PD098059, inhibitors of the P13-kinase and MAPK pathways, respectively. As revealed by confocal laser microscopy, both syntaxin 1A and synaptotagmin IV were immunolocated in islet cells, including the insulin-containing cells. These results indicate that PRL modulates the final steps of insulin secretion by increasing the expression of proteins involved in membrane fusion.
dc.description39
dc.description3
dc.description555
dc.description566
dc.languageen
dc.publisherSociedad Biolgia Chile
dc.publisherSantiago
dc.publisherChile
dc.relationBiological Research
dc.relationBiol. Res.
dc.rightsfechado
dc.sourceWeb of Science
dc.subjectinsulin
dc.subjectpancreatic islets
dc.subjectpregnant rats
dc.subjectprolactin
dc.subjectSNARE proteins
dc.subjectsynaptotagmin
dc.subjectPancreatic Beta-cells
dc.subjectInsulin-receptor Substrate-1
dc.subjectSyntaxin 1a
dc.subjectSulfonylurea Receptor-1
dc.subjectT-snare
dc.subjectExocytosis
dc.subjectSecretion
dc.subjectCalcium
dc.subjectChannel
dc.subjectDomain
dc.titleIncreased expression of SNARE proteins and synaptotagmin IV in islets from pregnant rats and in vitro prolactin-treated neonatal islets
dc.typeArtículos de revistas


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