The CATS (FAM64A) protein is a substrate of the Kinase Interacting Stathmin (KIS)

dc.creatorArchangelo, LF
dc.creatorGreif, PA
dc.creatorMaucuer, A
dc.creatorManceau, V
dc.creatorKoneru, N
dc.creatorBigarella, CL
dc.creatorNiemann, F
dc.creatordos Santos, MT
dc.creatorKobarg, J
dc.creatorBohlander, SK
dc.creatorSaad, STO
dc.date2013
dc.dateMAY
dc.date2014-08-01T18:23:16Z
dc.date2015-11-26T17:55:54Z
dc.date2014-08-01T18:23:16Z
dc.date2015-11-26T17:55:54Z
dc.date.accessioned2018-03-29T00:39:36Z
dc.date.available2018-03-29T00:39:36Z
dc.identifierBiochimica Et Biophysica Acta-molecular Cell Research. Elsevier Science Bv, v. 1833, n. 5, n. 1269, n. 1279, 2013.
dc.identifier0167-4889
dc.identifierWOS:000317374500031
dc.identifier10.1016/j.bbamcr.2013.02.004
dc.identifierhttp://www.repositorio.unicamp.br/jspui/handle/REPOSIP/78109
dc.identifierhttp://repositorio.unicamp.br/jspui/handle/REPOSIP/78109
dc.identifier.urihttp://repositorioslatinoamericanos.uchile.cl/handle/2250/1291132
dc.descriptionFundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)
dc.descriptionConselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)
dc.descriptionThe CATS protein (also known as FAM64A and RCS1) was first identified as a novel CALM (PICALM) interactor that influences the subcellular localization of the leukemogenic fusion protein CALM/AF10. CATS is highly expressed in cancer cell lines in a cell cycle dependent manner and is induced by mitogens. CATS is considered a marker for proliferation, known to control the metaphase-to-anaphase transition during the cell division. Using CATS as a bait in a yeast two-hybrid screen we identified the Kinase Interacting Stathmin (MS or UHMK1) protein as a CATS interacting partner. The interaction between CATS and KIS was confirmed by GST pull-down, co-immunopreciptation and co-localization experiments. Using kinase assay we showed that CATS is a substrate of KIS and mapped the phosphorylation site to CATS serine 131 (S131). Protein expression analysis revealed that KIS levels changed in a cell cycle-dependent manner and in the opposite direction to CATS levels. In a reporter gene assay KIS was able to enhance the transcriptional repressor activity of CATS, independent of CATS phophorylation at S131. Moreover, we showed that CATS and KIS antagonize the transactivation capacity of CALM/AF10.In summary, our results show that CATS interacts with and is a substrate for KIS, suggesting that KIS regulates CATS function. (c) 2013 Elsevier B.V. All rights reserved.
dc.description1833
dc.description5
dc.description1269
dc.description1279
dc.descriptionFundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)
dc.descriptionConselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)
dc.descriptionDeutsche Krebshilfe [109031]
dc.descriptionDeutsche Forschungsgemeinschaft [SFB 684 A6, BO 938/4-1]
dc.descriptionFundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)
dc.descriptionConselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)
dc.descriptionFAPESP [07/08019-1, 07/54870-5]
dc.descriptionCNPq [101624/2011-5]
dc.descriptionDeutsche Krebshilfe [109031]
dc.descriptionDeutsche Forschungsgemeinschaft [SFB 684 A6, BO 938/4-1]
dc.languageen
dc.publisherElsevier Science Bv
dc.publisherAmsterdam
dc.publisherHolanda
dc.relationBiochimica Et Biophysica Acta-molecular Cell Research
dc.relationBiochim. Biophys. Acta-Mol. Cell Res.
dc.rightsfechado
dc.rightshttp://www.elsevier.com/about/open-access/open-access-policies/article-posting-policy
dc.sourceWeb of Science
dc.subjectCATS
dc.subjectFAM64A
dc.subjectKIS
dc.subjectUHMK1
dc.subjectPhosphorylation
dc.subjectCALM/AF10-leukemia
dc.subjectAcute Myeloid-leukemia
dc.subjectRna-recognition Motif
dc.subjectQuantitative Phosphoproteomics
dc.subjectSubcellular-localization
dc.subjectUp-regulation
dc.subjectCell-cycle
dc.subjectT-all
dc.subjectFusion
dc.subjectPhosphorylation
dc.subjectCalm-af10
dc.titleThe CATS (FAM64A) protein is a substrate of the Kinase Interacting Stathmin (KIS)
dc.typeArtículos de revistas


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