Urea Amperometric Biosensors Based on Nanostructured Polypyrrole

dc.creatorMassafera, MP
dc.creatorde Torresi, SIC
dc.date2011
dc.dateNOV
dc.date2014-08-01T18:16:38Z
dc.date2015-11-26T17:55:44Z
dc.date2014-08-01T18:16:38Z
dc.date2015-11-26T17:55:44Z
dc.date.accessioned2018-03-29T00:39:29Z
dc.date.available2018-03-29T00:39:29Z
dc.identifierElectroanalysis. Wiley-blackwell, v. 23, n. 11, n. 2534, n. 2540, 2011.
dc.identifier1040-0397
dc.identifierWOS:000297311500006
dc.identifier10.1002/elan.201100239
dc.identifierhttp://www.repositorio.unicamp.br/jspui/handle/REPOSIP/76434
dc.identifierhttp://repositorio.unicamp.br/jspui/handle/REPOSIP/76434
dc.identifier.urihttp://repositorioslatinoamericanos.uchile.cl/handle/2250/1291102
dc.descriptionFundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)
dc.descriptionConselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)
dc.descriptionIn this work, we report the development of urea biosensors using macroporous polypyrrole as electrochemical transducer. Nanostructured polypyrrole was synthesized using monodispersed polystyrene spheres (460 nm) as template. Nanostructured urea biosensors were assembled after immobilization of urease (an enzyme that hydrolyses urea to ammonia) on macroporous polypyrrole, which was carried out using two different procedures: covalent attachment or layer-by-layer (lbl) deposition. Results show that lbl assembly of urease on macroporous polypyrrole gives rise to biosensors with improved detection kinetics (as demonstrated by higher I(max) values) and tunable sensitivities. Comparison with bulk urea biosensors previously described in the literature demonstrates that the use of nanostructured polypyrrole as sensing platform enhances biosensor's analytical performances, mainly due to the availability of more active sites for enzyme immobilization.
dc.description23
dc.description11
dc.description2534
dc.description2540
dc.descriptionFundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)
dc.descriptionConselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)
dc.descriptionINCT-Bioanalitica
dc.descriptionFundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)
dc.descriptionConselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)
dc.descriptionFAPESP [2009/53199-3]
dc.languageen
dc.publisherWiley-blackwell
dc.publisherMalden
dc.publisherEUA
dc.relationElectroanalysis
dc.relationElectroanalysis
dc.rightsfechado
dc.rightshttp://olabout.wiley.com/WileyCDA/Section/id-406071.html
dc.sourceWeb of Science
dc.subjectUrea
dc.subjectPolypyrrole
dc.subjectTemplate nanostructuration
dc.subjectBiosensors
dc.subjectElectrochemistry
dc.subjectSelf-assembly
dc.subjectDodecylbenzenesulfonate-doped Polypyrrole
dc.subjectQuartz-crystal Microbalance
dc.subjectIonic Exchanges
dc.subjectThin-films
dc.subjectAmmonia
dc.subjectLayer
dc.subjectMicrocontainers
dc.subjectNanocomposites
dc.subjectAdsorption
dc.subjectTemplates
dc.titleUrea Amperometric Biosensors Based on Nanostructured Polypyrrole
dc.typeArtículos de revistas


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