Titanium and zirconia particle-induced pro-inflammatory gene expression in cultured macrophages and osteolysis, inflammatory hyperalgesia and edema in vivo

dc.creatorObando-Pereda, GA
dc.creatorFischer, L
dc.creatorStach-Machado, DR
dc.date2014
dc.date37681
dc.date2014-08-01T18:22:24Z
dc.date2015-11-26T17:54:59Z
dc.date2014-08-01T18:22:24Z
dc.date2015-11-26T17:54:59Z
dc.date.accessioned2018-03-29T00:38:44Z
dc.date.available2018-03-29T00:38:44Z
dc.identifierLife Sciences. Pergamon-elsevier Science Ltd, v. 97, n. 2, n. 96, n. 106, 2014.
dc.identifier0024-3205
dc.identifier1879-0631
dc.identifierWOS:000331920800002
dc.identifier10.1016/j.lfs.2013.11.008
dc.identifierhttp://www.repositorio.unicamp.br/jspui/handle/REPOSIP/77957
dc.identifierhttp://repositorio.unicamp.br/jspui/handle/REPOSIP/77957
dc.identifier.urihttp://repositorioslatinoamericanos.uchile.cl/handle/2250/1290914
dc.descriptionFundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)
dc.descriptionAims: The biological reaction to wear debris is critical to the osteolysis underlying aseptic loosening of joint prosthetic implants. In an attempt to reduce aseptic loosening, ceramics have been introduced. This study was designed to evaluate, compare and correlate the expression of Toll-like receptors (TLRs), their intracellular adaptors and proinflammatory cytokines in cultured macrophages challenged with titanium or zirconia particles, as well as particle-induced osteolysis in calvaria and hyperalgesia and edema in hind paw. Main methods: TLRs and their adaptors were evaluated at the mRNA level by RT-PCR, and cytokine expression was evaluated at the mRNA and protein levels. Osteolysis and hyperalgesia and edema were evaluated in vivo, in calvaria and hind paw, respectively. Key findings: Cultured macrophages challenged with zirconia or titanium particles expressed increased mRNA for TLRs 2, 3, 4 and 9, and their adaptors MyD88, TR1F and NF-kappa B and cytokines TNF-alpha, IL-1 beta and 1L-6, which were also increased at protein level. Quantitative differences are evident and, in general, zirconia particle-induced pro-inflammatory gene expression was lower than that induced by titanium particles. In in vivo experiments, exposition to titanium or zirconia particles induced osteolysis in calvaria and hyperalgesia and edema in hind paw; however those induced by zirconia particles were significantly lower. There is a strong and positive correlation between the expressions of mRNA for TLR4, NF-kappa B, TNF-alpha, IL-1 beta and IL-6. Significance: Collectively, our data suggest that zirconia ceramic particles are less bioactive than titanium particles. (C) 2013 Elsevier Inc. All rights reserved.
dc.description97
dc.description2
dc.description96
dc.description106
dc.descriptionFundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)
dc.descriptionFundacao Araucaria, Governo do Estado do Parana, Brazil
dc.descriptionFundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)
dc.descriptionFAPESP [07/58796-4, 07/56731-2]
dc.languageen
dc.publisherPergamon-elsevier Science Ltd
dc.publisherOxford
dc.publisherInglaterra
dc.relationLife Sciences
dc.relationLife Sci.
dc.rightsfechado
dc.rightshttp://www.elsevier.com/about/open-access/open-access-policies/article-posting-policy
dc.sourceWeb of Science
dc.subjectZirconia particles
dc.subjectTitanium particles
dc.subjectAseptic loosening
dc.subjectToll-like receptors
dc.subjectCytokines
dc.subjectOsteolysis
dc.subjectMacrophage
dc.subjectToll-like Receptors
dc.subjectTotal Hip-arthroplasty
dc.subjectOsteoclast Differentiation
dc.subjectPeriprosthetic Osteolysis
dc.subjectPolyethylene Particles
dc.subjectCytokine Production
dc.subjectWear Particles
dc.subjectImplants
dc.subjectAlumina
dc.subjectVitro
dc.titleTitanium and zirconia particle-induced pro-inflammatory gene expression in cultured macrophages and osteolysis, inflammatory hyperalgesia and edema in vivo
dc.typeArtículos de revistas


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