The cholinergic response is increased in isolated ileum from gastroschisis rat model

dc.creatorRegis, AC
dc.creatorRojas-Moscoso, JA
dc.creatorGoncalves, FLL
dc.creatorSchmidt, AF
dc.creatorMonica, FZ
dc.creatorAntunes, E
dc.creatorSbragia, L
dc.date2011
dc.dateSEP
dc.date2014-07-31T13:53:29Z
dc.date2015-11-26T17:54:14Z
dc.date2014-07-31T13:53:29Z
dc.date2015-11-26T17:54:14Z
dc.date.accessioned2018-03-29T00:37:51Z
dc.date.available2018-03-29T00:37:51Z
dc.identifierPediatric Surgery International. Springer, v. 27, n. 9, n. 1015, n. 1019, 2011.
dc.identifier0179-0358
dc.identifierWOS:000293979400017
dc.identifier10.1007/s00383-011-2923-3
dc.identifierhttp://www.repositorio.unicamp.br/jspui/handle/REPOSIP/74946
dc.identifierhttp://repositorio.unicamp.br/jspui/handle/REPOSIP/74946
dc.identifier.urihttp://repositorioslatinoamericanos.uchile.cl/handle/2250/1290736
dc.descriptionFundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)
dc.descriptionCoordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)
dc.descriptionBabies with gastroschisis (G) have high morbidity rate and long hospital stay due to bowel hypomotility caused by chronic exposure of the bowel to the amniotic fluid. Our aim was to evaluate the reactivity of isolated ileum in fetal rats selected for experimental gastroschisis. G was surgically created at 18.5 days of gestation (term = 22 days). Concentration-dependent curve to the muscarinic agonist methacholine (1-30 mu M) and contractions induced by electrical field stimulation (EFS, 1-16 Hz, 50 V, 1 ms) were carried out in isolated ileum of groups control (C), sham (S) and gastroschisis (G) (n = 30). Protein expression for M(3) was assessed by western blot analysis. The frequency and amplitude of spontaneous contractions were decreased in G (p < 0.001). Methacholine produced concentration-dependent contractions being the maximal response values higher in G (p < 0.01). EFS-induced frequency-dependent contractions showed 1.8 times higher in G as well as an increase of M(3) expression. The frequency and the amplitude of rhythmic contractions were reduced along with an increase in the contraction induced by mucarinic agonist and by EFS in G. These results suggest the occurrence of an adaptative supersensitivity to cholinergic response via increases in the protein expression for M(3) receptor.
dc.description27
dc.description9
dc.description1015
dc.description1019
dc.descriptionFundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)
dc.descriptionCoordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)
dc.descriptionFundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)
dc.descriptionCoordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)
dc.descriptionFAPESP [08/51487-9]
dc.languageen
dc.publisherSpringer
dc.publisherNew York
dc.publisherEUA
dc.relationPediatric Surgery International
dc.relationPediatr. Surg. Int.
dc.rightsfechado
dc.rightshttp://www.springer.com/open+access/authors+rights?SGWID=0-176704-12-683201-0
dc.sourceWeb of Science
dc.subjectGastroschisis
dc.subjectRat model
dc.subjectIntestinal motility
dc.subjectFetal surgery
dc.subjectGut development
dc.subjectEnteric Nervous-system
dc.subjectSmall-bowel Obstruction
dc.subjectInterstitial-cells
dc.subjectC-kit
dc.subjectIntestinal Damage
dc.subjectDelayed Maturation
dc.subjectChick-embryos
dc.subjectSmooth-muscle
dc.subjectRabbit Model
dc.subjectFetal
dc.titleThe cholinergic response is increased in isolated ileum from gastroschisis rat model
dc.typeArtículos de revistas


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