Rapid differentiation among bacteria that cause gastroenteritis by use of low-resolution Raman spectroscopy and PLS discriminant analysis

dc.creatorMello, C
dc.creatorRibeiro, D
dc.creatorNovaes, F
dc.creatorPoppi, RJ
dc.date2005
dc.dateOCT
dc.date2014-11-18T19:14:27Z
dc.date2015-11-26T17:53:19Z
dc.date2014-11-18T19:14:27Z
dc.date2015-11-26T17:53:19Z
dc.date.accessioned2018-03-29T00:36:53Z
dc.date.available2018-03-29T00:36:53Z
dc.identifierAnalytical And Bioanalytical Chemistry. Springer Heidelberg, v. 383, n. 4, n. 701, n. 706, 2005.
dc.identifier1618-2642
dc.identifierWOS:000233457900021
dc.identifier10.1007/s00216-005-0017-6
dc.identifierhttp://www.repositorio.unicamp.br/jspui/handle/REPOSIP/58890
dc.identifierhttp://www.repositorio.unicamp.br/handle/REPOSIP/58890
dc.identifierhttp://repositorio.unicamp.br/jspui/handle/REPOSIP/58890
dc.identifier.urihttp://repositorioslatinoamericanos.uchile.cl/handle/2250/1290492
dc.descriptionUse of classical microbiological methods to differentiate bacteria that cause gastroenteritis is cumbersome but usually very efficient. The high cost of reagents and the time required for such identifications, approximately four days, could have serious consequences, however, mainly when the patients are children, the elderly, or adults with low resistance. The search for new methods enabling rapid and reagentless differentiation of these microorganisms is, therefore, extremely relevant. In this work the main microorganisms responsible for gastroenteritis, Escherichia coli, Salmonella choleraesuis, and Shigella flexneri, were studied. For each microorganism sixty different dispersions were prepared in physiological solution. The Raman spectra of these dispersions were recorded using a diode laser operating in the near infrared region. Partial least-squares (PLS) discriminant analysis was used to differentiate among the bacteria by use of their respective Raman spectra. This approach enabled correct classification of 100% of the bacteria evaluated and unknown samples from the clinical environment, in less time (similar to 10 h), by use of a low-cost, portable Raman spectrometer, which can be easily used in intensive care units and clinical environments.
dc.description383
dc.description4
dc.description701
dc.description706
dc.languageen
dc.publisherSpringer Heidelberg
dc.publisherHeidelberg
dc.publisherAlemanha
dc.relationAnalytical And Bioanalytical Chemistry
dc.relationAnal. Bioanal. Chem.
dc.rightsfechado
dc.rightshttp://www.springer.com/open+access/authors+rights?SGWID=0-176704-12-683201-0
dc.sourceWeb of Science
dc.subjectPLS discriminant analysis
dc.subjectraman spectroscopy
dc.subjectbacteria
dc.subjectgastroenteritis
dc.subjectAmide-i Modes
dc.subjectVibrational Spectroscopy
dc.subjectIdentification
dc.subjectTransform
dc.titleRapid differentiation among bacteria that cause gastroenteritis by use of low-resolution Raman spectroscopy and PLS discriminant analysis
dc.typeArtículos de revistas


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