Increase in probing depth is correlated with a higher number of Prevotella intermedia genotypes

dc.creatorAlves, ACBA
dc.creatorNapimoga, MH
dc.creatorKlein, MI
dc.creatorHofling, JF
dc.creatorGoncalves, RB
dc.date2006
dc.dateJAN
dc.date2014-11-18T18:45:12Z
dc.date2015-11-26T17:53:15Z
dc.date2014-11-18T18:45:12Z
dc.date2015-11-26T17:53:15Z
dc.date.accessioned2018-03-29T00:36:49Z
dc.date.available2018-03-29T00:36:49Z
dc.identifierJournal Of Periodontology. Amer Acad Periodontology, v. 77, n. 1, n. 61, n. 66, 2006.
dc.identifier0022-3492
dc.identifierWOS:000241878100009
dc.identifier10.1902/jop.2006.77.1.61
dc.identifierhttp://www.repositorio.unicamp.br/jspui/handle/REPOSIP/59998
dc.identifierhttp://www.repositorio.unicamp.br/handle/REPOSIP/59998
dc.identifierhttp://repositorio.unicamp.br/jspui/handle/REPOSIP/59998
dc.identifier.urihttp://repositorioslatinoamericanos.uchile.cl/handle/2250/1290477
dc.descriptionBackground: The aims of this study were to determine the genotypic diversity of Prevotella intermedia in subgingival plaque samples by using two techniques, arbitrarily primed polymerase chain reaction (AP-PCR) and heteroduplex analysis, and to assess the relationship of this diversity with increase in probing depth. Methods: The subgingival plaque samples were obtained from 12 patients using paper points inserted into periodontal pockets (diseased sites) and healthy gingival sulci (healthy sites) of the same subjects. After isolation and identification, AP-PCR was performed for genotypic characterization of P. intermedia (80 isolates). The clinical samples with a positive result for P. intermedia were amplified by 16S rRNA-based PCR method, and the amplicons were subjected to heteroduplex analysis. Results: The agreement between the two methods was very high; the AP-PCR and heteroduplex analysis showed that subjects harbored between one and five distinct genotypes of P. intermedia, with a positive association between numbers of genotypes by AP-PCR (P = 0.0042) or heteroduplex (P = 0.0099) and increase in probing depth. No matching of P. intermedia genotypes was observed between healthy and diseased sites of the same individual. Interindividual analyses demonstrated absence of identical clones and indicated a high level of genetic diversity in the species. Conclusion: A clear relationship was observed between a higher number of genotypes and increase in probing depth; these results suggest that environmental challenges in the periodontal pockets may modulate the microbiota by selecting genotypes best able to exploit the environment.
dc.description77
dc.description1
dc.description61
dc.description66
dc.languageen
dc.publisherAmer Acad Periodontology
dc.publisherChicago
dc.publisherEUA
dc.relationJournal Of Periodontology
dc.relationJ. Periodont.
dc.rightsfechado
dc.sourceWeb of Science
dc.subjectgenotypes
dc.subjectheteroduplex
dc.subjectperiodontal disease
dc.subjectpolymerase chain reaction
dc.subjectPrevotella intermedia
dc.subjectPolymerase-chain-reaction
dc.subjectAmplified Polymorphic Dna
dc.subjectPorphyromonas-gingivalis
dc.subjectStreptococcus-mutans
dc.subjectClonal Diversity
dc.subjectRoot Canals
dc.subjectNigrescens
dc.subjectPeriodontitis
dc.subjectPlaque
dc.subjectMicroorganisms
dc.titleIncrease in probing depth is correlated with a higher number of Prevotella intermedia genotypes
dc.typeArtículos de revistas


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