Standardization of bulb and root sample sizes for the Allium cepa test

dc.creatorBarberio, A
dc.creatorVoltolini, JC
dc.creatorMello, MLS
dc.date2011
dc.dateJUN
dc.date2014-07-30T20:02:42Z
dc.date2015-11-26T17:52:48Z
dc.date2014-07-30T20:02:42Z
dc.date2015-11-26T17:52:48Z
dc.date.accessioned2018-03-29T00:36:21Z
dc.date.available2018-03-29T00:36:21Z
dc.identifierEcotoxicology. Springer, v. 20, n. 4, n. 927, n. 935, 2011.
dc.identifier0963-9292
dc.identifierWOS:000290330100029
dc.identifier10.1007/s10646-011-0602-8
dc.identifierhttp://www.repositorio.unicamp.br/jspui/handle/REPOSIP/74536
dc.identifierhttp://repositorio.unicamp.br/jspui/handle/REPOSIP/74536
dc.identifier.urihttp://repositorioslatinoamericanos.uchile.cl/handle/2250/1290356
dc.descriptionConselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)
dc.descriptionAlthough the Allium cepa test has been widely used to identify potentially cytotoxic and genotoxic pollutants in aquatic environments, variable non-standardized choices have been made regarding the number of plant bulbs and roots analyzed. We propose numbers for bulbs and roots per bulb when comparing the frequencies of micronuclei, mitotic anomalies and mitotic index with this test. Roots that had been treated with aqueous solutions, such as water samples collected in August 2007 from the Paraiba do Sul River at the Brazilian cities of Trememb, and Aparecida; negative and positive controls were used for bioassays. The presence of pollutants in the river water had been presumed based on our previous cytological data and an official report by the So Paulo State Environmental Agency (Brazil) on presence of fecal contaminants (Trememb, and Aparecida) and elevated dissolved aluminium (Aparecida) in the water under study. The sampling of ten bulbs and five roots per bulb was found adequate for comparative studies to evaluate with the A. cepa test the potential damage inflicted by pollutants in aquatic environments. Furthermore, even one bulb and one root per bulb was sufficient in discerning this damage, thereby shortening the time required to attain a statistically confident comparative evaluation. However, to allow for the use of statistical programs based on the evaluation of average values, and to avoid criticism based on genetic variability, we propose that three bulbs and three roots per bulb be considered as standard sample sizes for the A. cepa test.
dc.description20
dc.description4
dc.description927
dc.description935
dc.descriptionUniversity of Taubate
dc.descriptionConselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)
dc.descriptionConselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)
dc.descriptionCNPq [-PQ-1A]
dc.languageen
dc.publisherSpringer
dc.publisherDordrecht
dc.publisherHolanda
dc.relationEcotoxicology
dc.relationEcotoxicology
dc.rightsfechado
dc.rightshttp://www.springer.com/open+access/authors+rights?SGWID=0-176704-12-683201-0
dc.sourceWeb of Science
dc.subjectAllium cepa test
dc.subjectSample size
dc.subjectBulbs
dc.subjectRoots
dc.subjectMicronuclei
dc.subjectMitotic abnormalities
dc.subjectChromosome-aberration Assay
dc.subjectWaste-water
dc.subjectMaleic Hydrazide
dc.subjectMeristem Cells
dc.subjectMicronucleus Assays
dc.subjectMedicinal-plants
dc.subjectGenotoxicity
dc.subjectToxicity
dc.subjectClastogenicity
dc.subjectMechanisms
dc.titleStandardization of bulb and root sample sizes for the Allium cepa test
dc.typeArtículos de revistas


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