Simultaneous determination of nitrite and nitrate anions in plasma, urine and cell culture supernatants by high-performance liquid chromatography with post-column reactions

dc.creatorMuscara, MN
dc.creatordeNucci, G
dc.date1996
dc.date42309
dc.date2014-07-30T19:57:49Z
dc.date2015-11-26T17:52:23Z
dc.date2014-07-30T19:57:49Z
dc.date2015-11-26T17:52:23Z
dc.date.accessioned2018-03-29T00:35:51Z
dc.date.available2018-03-29T00:35:51Z
dc.identifierJournal Of Chromatography B-biomedical Applications. Elsevier Science Bv, v. 686, n. 2, n. 157, n. 164, 1996.
dc.identifier0378-4347
dc.identifierWOS:A1996VW93300005
dc.identifier10.1016/S0378-4347(96)00229-0
dc.identifierhttp://www.repositorio.unicamp.br/jspui/handle/REPOSIP/74210
dc.identifierhttp://repositorio.unicamp.br/jspui/handle/REPOSIP/74210
dc.identifier.urihttp://repositorioslatinoamericanos.uchile.cl/handle/2250/1290234
dc.descriptionA high-performance liquid chromatographic method for the determination of nitrite and nitrate anions derived from nitric oxide in biological fluids is presented. After separation on a strong anion-exchange column (Spherisorb SAX, 250x4.6 mm I.D., 5 mu m), two on-line post-column reactions occur. The first involves nitrate reduction to nitrite on a copper-plated cadmium-filled column. In the second, the diazotization-coupling reaction between nitrite and the Griess reagent (0.05% naphtylethylendiamine dihydrochloride plus 0.5% sulphanilamide in 5% phosphoric acid) takes place, and the absorbance of the chromophore is read at 540 nm. This methodology was applied to biological fluids. Before injection into the chromatographic system, the samples were diluted and submitted to suitable clean-up procedures (urine and cell culture supernatant samples are passed through C-18 cartridges, and serum samples were deproteinized by ultrafiltration through membranes with a molecular mass cut-off of 3000). The method has a sensitivity of 30 pmol for both anions, as little as 0.05-0.1 ml sample volume is required and linearity is observed up to 60 nmol for each anion.
dc.description686
dc.description2
dc.description157
dc.description164
dc.languageen
dc.publisherElsevier Science Bv
dc.publisherAmsterdam
dc.publisherHolanda
dc.relationJournal Of Chromatography B-biomedical Applications
dc.relationJ. Chromatogr. B-Biomed. Appl.
dc.rightsfechado
dc.rightshttp://www.elsevier.com/about/open-access/open-access-policies/article-posting-policy
dc.sourceWeb of Science
dc.subjectnitrite
dc.subjectnitrate
dc.subjectnitric oxide
dc.subjectIon Chromatography
dc.subjectGas-chromatography
dc.subjectFlow-injection
dc.subjectEndothelial-cells
dc.subjectRelaxing Factor
dc.subjectOxide Synthase
dc.subjectL-arginine
dc.subjectPlatelet-aggregation
dc.subjectMass-spectrometry
dc.subjectBiological-fluids
dc.titleSimultaneous determination of nitrite and nitrate anions in plasma, urine and cell culture supernatants by high-performance liquid chromatography with post-column reactions
dc.typeArtículos de revistas


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