Placenta cryosections for study of the adhesion of Plasmodium falciparum-infected erythrocytes to chondroitin sulfate A in flow conditions

dc.creatorAvril, M
dc.creatorTraore, B
dc.creatorCosta, FTM
dc.creatorLepolard, C
dc.creatorGysin, J
dc.date2004
dc.dateMAR
dc.date2014-11-18T15:11:49Z
dc.date2015-11-26T17:51:26Z
dc.date2014-11-18T15:11:49Z
dc.date2015-11-26T17:51:26Z
dc.date.accessioned2018-03-29T00:34:49Z
dc.date.available2018-03-29T00:34:49Z
dc.identifierMicrobes And Infection. Elsevier Science Bv, v. 6, n. 3, n. 249, n. 255, 2004.
dc.identifier1286-4579
dc.identifierWOS:000220392100001
dc.identifier10.1016/j.micinf.2003.12.007
dc.identifierhttp://www.repositorio.unicamp.br/jspui/handle/REPOSIP/59875
dc.identifierhttp://www.repositorio.unicamp.br/handle/REPOSIP/59875
dc.identifierhttp://repositorio.unicamp.br/jspui/handle/REPOSIP/59875
dc.identifier.urihttp://repositorioslatinoamericanos.uchile.cl/handle/2250/1289977
dc.descriptionThe adhesion of Plasmodium falciparum-infected erythrocytes (IEs) to chondroitin-4-sulfate (CSA) via the PfEMPI-CSA parasite ligand domain is correlated with placental malaria in primigravidae. The recent identification of parasite genes encoding CSA adhesion molecules and the development of pan-reactive monoclonal antibodies against the pf(CSA) ligand have opened up new avenues for the development of anti-IE sequestration therapies for the prevention of placental malaria. A model closely mimicking placental sequestration of IEs during pregnancy is needed for the preclinical and clinical evaluation of candidate molecules for the induction of antibodies that could protect pregnant women from placental malaria. We found that normal placenta cryosections were a specific and highly consistent support for the binding of IEs to CSA in flow conditions under physiological conditions. This model makes possible the quantitative and qualitative analysis of IE adhesion. We identified distinct CSA-binding phenotypes within the FCR3(CSA)-selected parasites in flow analyses, but not in static analyses. We also analyzed inhibitors of placental parasite binding such as soluble CSA and antibodies directed against the pf(CSA) ligand. Our data demonstrate that placenta cryosections could be used to standardize assays between laboratories, potentially advancing the development of therapies against placental malaria. (C) 2003 Elsevier SAS. All rights reserved.
dc.description6
dc.description3
dc.description249
dc.description255
dc.languageen
dc.publisherElsevier Science Bv
dc.publisherAmsterdam
dc.publisherHolanda
dc.relationMicrobes And Infection
dc.relationMicrobes Infect.
dc.rightsfechado
dc.rightshttp://www.elsevier.com/about/open-access/open-access-policies/article-posting-policy
dc.sourceWeb of Science
dc.subjectPlasmodium falciparum
dc.subjectcytoadhesion
dc.subjectinhibitors
dc.subjectchondroitin sulfate A
dc.subjectplacenta model
dc.subjectflow conditions
dc.subjectMicrovascular Endothelial-cells
dc.subjectRed-blood-cells
dc.subjectSaimiri-sciureus
dc.subjectMalaria
dc.subjectCytoadhesion
dc.subjectAntibodies
dc.subjectReceptor
dc.subjectSequestration
dc.subjectProteoglycan
dc.subjectAdherence
dc.titlePlacenta cryosections for study of the adhesion of Plasmodium falciparum-infected erythrocytes to chondroitin sulfate A in flow conditions
dc.typeArtículos de revistas


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