Inhibition of human pulpal gelatinases (MMP-2 and MMP-9) by zinc oxide cements

dc.creatorSantos, MCLG
dc.creatorDe Souza, AP
dc.creatorGerlach, RF
dc.creatorTrevilatto, PC
dc.creatorScarel-Caminaga, RM
dc.creatorLine, SRP
dc.date2004
dc.dateJUL
dc.date2014-11-18T13:01:42Z
dc.date2015-11-26T17:50:22Z
dc.date2014-11-18T13:01:42Z
dc.date2015-11-26T17:50:22Z
dc.date.accessioned2018-03-29T00:33:35Z
dc.date.available2018-03-29T00:33:35Z
dc.identifierJournal Of Oral Rehabilitation. Blackwell Publishing Ltd, v. 31, n. 7, n. 660, n. 664, 2004.
dc.identifier0305-182X
dc.identifierWOS:000222207600007
dc.identifier10.1111/j.1365-2842.2004.01297.x
dc.identifierhttp://www.repositorio.unicamp.br/jspui/handle/REPOSIP/80583
dc.identifierhttp://www.repositorio.unicamp.br/handle/REPOSIP/80583
dc.identifierhttp://repositorio.unicamp.br/jspui/handle/REPOSIP/80583
dc.identifier.urihttp://repositorioslatinoamericanos.uchile.cl/handle/2250/1289660
dc.descriptionThe interaction between metal ions and the oral environment is a major subject matter in dental research. Matrix metalloproteinases (MMPs) have been implicated in several pathological and physiological processes such as, periodontal tissue destruction, root caries, dentin calcification and pulpal inflammation. The aim of this work was to test the effect of zinc released from zinc oxide-eugenol (ZOE) cements, on the activity of the major pulpal gelatinolytic MMPs. Pulpal explants were cultured overnight in Dulbecco's Modified Eagle Medium and the activity of secreted enzymes was analysed by gelatin zymography in buffer conditionated with diverse ZOE cements. Phenanthroline, a zinc chelator, was used to revert the inhibition of MMPs caused by zinc. The major gelatinolytic proteinases present in the conditioned media were characterized as MMP-2 and MMP-9 by immunoprecipitation. All ZOE cements inhibited MMPs activity, whereas phenanthroline could partially revert the inhibition caused by plain ZOE and Intermediate Restorative Material (IRM).
dc.description31
dc.description7
dc.description660
dc.description664
dc.languageen
dc.publisherBlackwell Publishing Ltd
dc.publisherOxford
dc.publisherInglaterra
dc.relationJournal Of Oral Rehabilitation
dc.relationJ. Oral Rehabil.
dc.rightsfechado
dc.sourceWeb of Science
dc.subjectzinc oxide-eugenol
dc.subjectmatrix metalloproteinase-2
dc.subjectmatrix metalloproteinase-9
dc.subjectmetalloproteinase
dc.subjectSoft Connective-tissue
dc.subjectMatrix Metalloproteinases
dc.subjectDentin Matrix
dc.subjectEugenol
dc.subjectCytotoxicity
dc.subjectActivation
dc.subjectCells
dc.subjectDegradation
dc.subjectCollagenase
dc.subjectFibroblasts
dc.titleInhibition of human pulpal gelatinases (MMP-2 and MMP-9) by zinc oxide cements
dc.typeArtículos de revistas


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