PIP4KIIA and beta-globin: transcripts differentially expressed in reticulocytes and associated with high levels of Hb H in two siblings with Hb H disease

dc.creatorWenning, MRSC
dc.creatorMello, MP
dc.creatorAndrade, TG
dc.creatorLanaro, C
dc.creatorAlbuquerque, DM
dc.creatorSaad, STO
dc.creatorCosta, FF
dc.creatorSonati, MF
dc.date2009
dc.dateNOV
dc.date2014-11-18T12:36:07Z
dc.date2015-11-26T17:50:16Z
dc.date2014-11-18T12:36:07Z
dc.date2015-11-26T17:50:16Z
dc.date.accessioned2018-03-29T00:33:27Z
dc.date.available2018-03-29T00:33:27Z
dc.identifierEuropean Journal Of Haematology. Wiley-blackwell, v. 83, n. 5, n. 490, n. 493, 2009.
dc.identifier0902-4441
dc.identifierWOS:000270650700012
dc.identifier10.1111/j.1600-0609.2009.01325.x
dc.identifierhttp://www.repositorio.unicamp.br/jspui/handle/REPOSIP/59857
dc.identifierhttp://www.repositorio.unicamp.br/handle/REPOSIP/59857
dc.identifierhttp://repositorio.unicamp.br/jspui/handle/REPOSIP/59857
dc.identifier.urihttp://repositorioslatinoamericanos.uchile.cl/handle/2250/1289630
dc.descriptionFundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)
dc.descriptionWe are reporting here the results of differential gene expression experiments comparing two siblings, a 21-yr-old male and a 19-yr-old female, with the same alpha-thalassemia genotype (-alpha 3.7/--SEA) and quite different levels of Hb H in the peripheral blood (18.7 and 5%, respectively). By using mRNA differential-display reverse-transcription-PCR and suppression subtractive hybridization, two main transcripts were selected in both procedures and validated by qRT-PCR, one corresponding to the phosphatidylinositol phosphate 4-kinase type II-alpha (PIP4KIIA) gene and the other to the beta-globin gene, both over expressed in the patient with the higher percentage of Hb H. Type II PIP kinases produce phosphatidylinositol 4,5 biphosphate, a critical and pleiotropic regulatory molecule involved in diverse cellular activities, including gene expression. Our results suggest that PIP4KIIA may be one of the factors related to the regulation of the beta-globin gene expression and the different levels of Hb H in alpha-thalassemic patients.
dc.description83
dc.description5
dc.description490
dc.description493
dc.descriptionFundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)
dc.descriptionFundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)
dc.descriptionFAPESP [02/13801-7]
dc.languageen
dc.publisherWiley-blackwell
dc.publisherMalden
dc.publisherEUA
dc.relationEuropean Journal Of Haematology
dc.relationEur. J. Haematol.
dc.rightsfechado
dc.rightshttp://olabout.wiley.com/WileyCDA/Section/id-406071.html
dc.sourceWeb of Science
dc.subjectPIP kinase
dc.subjectglobin genes
dc.subjectalpha-thalassemia
dc.subjectHb H disease
dc.subjectdifferential gene expression
dc.subjectPhosphatidylinositol Phosphate Kinases
dc.subjectFamily
dc.titlePIP4KIIA and beta-globin: transcripts differentially expressed in reticulocytes and associated with high levels of Hb H in two siblings with Hb H disease
dc.typeArtículos de revistas


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