The role of transient receptor potential A 1 (TRPA1) in the development and maintenance of carrageenan-induced hyperalgesia

dc.creatorBonet, IJM
dc.creatorFischer, L
dc.creatorParada, CA
dc.creatorTambeli, CH
dc.date2013
dc.dateFEB
dc.date2014-07-30T19:00:21Z
dc.date2015-11-26T17:50:14Z
dc.date2014-07-30T19:00:21Z
dc.date2015-11-26T17:50:14Z
dc.date.accessioned2018-03-29T00:33:25Z
dc.date.available2018-03-29T00:33:25Z
dc.identifierNeuropharmacology. Pergamon-elsevier Science Ltd, v. 65, n. 206, n. 212, 2013.
dc.identifier0028-3908
dc.identifierWOS:000313935300026
dc.identifier10.1016/j.neuropharm.2012.09.020
dc.identifierhttp://www.repositorio.unicamp.br/jspui/handle/REPOSIP/72414
dc.identifierhttp://repositorio.unicamp.br/jspui/handle/REPOSIP/72414
dc.identifier.urihttp://repositorioslatinoamericanos.uchile.cl/handle/2250/1289619
dc.descriptionTransient receptor potential ankyrin 1 (TRPA1) is a nonselective cation channel important in setting nociceptive threshold. It is expressed in nociceptive C-fibers and in non-neuronal cells involved in pro-inflammatory mediators' release. We asked whether TRPA1 contributes to carrageenan-induced hyperalgesia in rats, and if so, whether this contribution is mediated by mechanisms involved in inflammation such as cytokine release and neutrophil migration and/or by a direct sensitization of the primary afferent nociceptors. Pharmacological blockade of local TRPA1 by its selective antagonist HC 030031 prevented and reversed carrageenan-induced hyperalgesia, which was detected either by a mechanical or chemical (low dose of capsaicin) stimulus. However, it did not affect either carrageenan-induced cytokines expression or neutrophil migration. The neuronal TRPA1 gene silencing induced by intrathecal pretreatment with antisense oligodoexynucleotide completely prevented carrageenan-induced hyperalgesia over 24 h and significantly reduced TRPA1 expression in the dorsal root ganglia cells (L5-6), which was not affected by carrageenan treatment. We conclude that TRPA1 plays an important role in the development and maintenance of carrageenan-induced inflammatory hyperalgesia by directly contributing to nociceptor excitability. (C) 2012 Elsevier Ltd. All rights reserved.
dc.description65
dc.description206
dc.description212
dc.languageen
dc.publisherPergamon-elsevier Science Ltd
dc.publisherOxford
dc.publisherInglaterra
dc.relationNeuropharmacology
dc.relationNeuropharmacology
dc.rightsfechado
dc.rightshttp://www.elsevier.com/about/open-access/open-access-policies/article-posting-policy
dc.sourceWeb of Science
dc.subjectTRPA1
dc.subjectCarrageenan
dc.subjectNociceptor sensitization
dc.subjectHyperalgesia
dc.subjectInflammation
dc.subjectCytokines
dc.subjectNeutrophil migration
dc.subjectGonadal-steroid Hormones
dc.subjectTemporomandibular-joint
dc.subjectInflammatory Hyperalgesia
dc.subjectMechanical Hyperalgesia
dc.subjectSensory Nerves
dc.subjectMast-cells
dc.subjectActivation
dc.subjectExpression
dc.subjectNeurons
dc.subjectRat
dc.titleThe role of transient receptor potential A 1 (TRPA1) in the development and maintenance of carrageenan-induced hyperalgesia
dc.typeArtículos de revistas


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