Fluoro-Jade, but not Fluoro-Jade B, stains non-degenerating cells in brain and retina of embryonic and neonatal rats

dc.creatorFernandes, AMAP
dc.creatorMaurer-Morelli, CV
dc.creatorCampos, CBL
dc.creatorMello, MLS
dc.creatorCastilho, RF
dc.creatorLangone, F
dc.date2004
dc.dateDEC 10
dc.date2014-11-18T10:09:26Z
dc.date2015-11-26T17:49:18Z
dc.date2014-11-18T10:09:26Z
dc.date2015-11-26T17:49:18Z
dc.date.accessioned2018-03-29T00:32:21Z
dc.date.available2018-03-29T00:32:21Z
dc.identifierBrain Research. Elsevier Science Bv, v. 1029, n. 1, n. 24, n. 33, 2004.
dc.identifier0006-8993
dc.identifierWOS:000225481700003
dc.identifier10.1016/j.brainres.2004.09.036
dc.identifierhttp://www.repositorio.unicamp.br/jspui/handle/REPOSIP/67348
dc.identifierhttp://www.repositorio.unicamp.br/handle/REPOSIP/67348
dc.identifierhttp://repositorio.unicamp.br/jspui/handle/REPOSIP/67348
dc.identifier.urihttp://repositorioslatinoamericanos.uchile.cl/handle/2250/1289343
dc.descriptionFluoro-Jade (FJ) and Fluoro-Jade B (FJB) are fluorescein derivatives currently used to stain brain cells under degeneration. In this study, we investigated the FJ staining of nondegenerating cells in embryonic and neonatal rat brain and retina. In embryonic rat brain (embryonic day 15; E15), very intense staining of cells was observed. The number of FJ-stained cells and the intensity of staining decreased with increasing in animal age, being almost absent by postnatal day 16 (P16). Only a few cells in neonatal rat brain were in the process of cell death, as verified by the TUNEL technique. The H-stained cells in neonatal brain were positive for the neuronal marker neuronal nuclei antigen (NeuN). In retina, FJ stained mainly cells from the ganglion cell layer at P2 and the neuroblastic layer at P2 and P6. In contrast to FJ, FJB did not stain nondegenerating cells in embryonic and neonatal rats. These results show that in addition to staining degenerating brain cells, FJ also stains nondegenerating central nervous system cells in embryonic and neonatal stages. (C) 2004 Elsevier B.V. All rights reserved.
dc.description1029
dc.description1
dc.description24
dc.description33
dc.languageen
dc.publisherElsevier Science Bv
dc.publisherAmsterdam
dc.publisherHolanda
dc.relationBrain Research
dc.relationBrain Res.
dc.rightsfechado
dc.rightshttp://www.elsevier.com/about/open-access/open-access-policies/article-posting-policy
dc.sourceWeb of Science
dc.subjectcell death
dc.subjectdevelopment
dc.subjectFluoro-Jade
dc.subjectFluoro-Jade B
dc.subjectneurodegeneration
dc.subjectneuron
dc.subjectInduced Striatal Excitotoxicity
dc.subjectNeuronal Degeneration
dc.subjectSlice Cultures
dc.subjectCerebral-cortex
dc.subjectNeurodegeneration
dc.subjectInjury
dc.subjectMouse
dc.subjectDeath
dc.subjectVulnerability
dc.subjectNeurotransmitter
dc.titleFluoro-Jade, but not Fluoro-Jade B, stains non-degenerating cells in brain and retina of embryonic and neonatal rats
dc.typeArtículos de revistas


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