EXPANDED BED ADSORPTION OF BROMELAIN (EC 3.4.22.33) FROM Ananas comosus CRUDE EXTRACT

dc.creatorSilveira, E
dc.creatorSouza, ME
dc.creatorSantana, JCC
dc.creatorChaves, AC
dc.creatorPorto, ALF
dc.creatorTambourgi, EB
dc.date2009
dc.dateJAN-MAR
dc.date2014-11-18T09:58:30Z
dc.date2015-11-26T17:49:15Z
dc.date2014-11-18T09:58:30Z
dc.date2015-11-26T17:49:15Z
dc.date.accessioned2018-03-29T00:32:17Z
dc.date.available2018-03-29T00:32:17Z
dc.identifierBrazilian Journal Of Chemical Engineering. Brazilian Soc Chemical Eng, v. 26, n. 1, n. 149, n. 157, 2009.
dc.identifier0104-6632
dc.identifierWOS:000264200500014
dc.identifierhttp://www.repositorio.unicamp.br/jspui/handle/REPOSIP/66320
dc.identifierhttp://www.repositorio.unicamp.br/handle/REPOSIP/66320
dc.identifierhttp://repositorio.unicamp.br/jspui/handle/REPOSIP/66320
dc.identifier.urihttp://repositorioslatinoamericanos.uchile.cl/handle/2250/1289326
dc.descriptionCoordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)
dc.descriptionConselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)
dc.descriptionThis work focuses on the adsorption of Bromelain in expanded bed conditions, such as the adsorption kinetics parameters. The adsorption kinetics parameters showed that after 40 minutes equilibrium was achieved and maximum adsorption capacity was 6.11 U per resin mL. However, the maximum adsorption capacity was only determined by measuring the adsorption isotherm. Only by the Langmuir model the maximum adsorption capacity, Qm, and dissociation constant, kd, values could be estimated as 9.18 U/mL and 0.591, respectively, at 25 degrees C and 0.1 mol/L phosphate buffer pH 7.5. A column made of glass with an inner diameter of 1 cm was used for the expanded bed adsorption (EBA). The residence time was reduced 10 fold by increasing the expansion degree 2.5 times; nonetheless, the plate number (N) value was reduced only 2 fold. After adsorption, the bromelain was eluted in packed bed mode, with a downward flow. The purification factor was about 13 fold and the total protein was reduced 4 fold. EBA showed to be feasible for purification of bromelain.
dc.description26
dc.description1
dc.description149
dc.description157
dc.descriptionCoordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)
dc.descriptionConselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)
dc.descriptionCoordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)
dc.descriptionConselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)
dc.languageen
dc.publisherBrazilian Soc Chemical Eng
dc.publisherSao Paulo
dc.publisherBrasil
dc.relationBrazilian Journal Of Chemical Engineering
dc.relationBraz. J. Chem. Eng.
dc.rightsaberto
dc.sourceWeb of Science
dc.subjectAdsorption
dc.subjectEnzyme Activity
dc.subjectPurification
dc.subjectAxial Dispersion
dc.subjectExpanded Bed Adsorption
dc.subjectBromelain
dc.subjectDeglycosylated Stem Bromelain
dc.subjectPineapple Stem
dc.subjectPurification
dc.subjectChromatography
dc.subjectDistributions
dc.subjectComponents
dc.subjectParticles
dc.subjectProteins
dc.subjectBehavior
dc.subjectSystems
dc.titleEXPANDED BED ADSORPTION OF BROMELAIN (EC 3.4.22.33) FROM Ananas comosus CRUDE EXTRACT
dc.typeArtículos de revistas


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