Role of Glucosyltransferase B in Interactions of Candida albicans with Streptococcus mutans and with an Experimental Pellicle on Hydroxyapatite Surfaces

dc.creatorGregoire, S
dc.creatorXiao, J
dc.creatorSilva, BB
dc.creatorGonzalez, I
dc.creatorAgidi, PS
dc.creatorKlein, MI
dc.creatorAmbatipudi, KS
dc.creatorRosalen, PL
dc.creatorBauserman, R
dc.creatorWaugh, RE
dc.creatorKoo, H
dc.date2011
dc.dateSEP
dc.date2014-07-30T18:28:14Z
dc.date2015-11-26T17:49:13Z
dc.date2014-07-30T18:28:14Z
dc.date2015-11-26T17:49:13Z
dc.date.accessioned2018-03-29T00:32:14Z
dc.date.available2018-03-29T00:32:14Z
dc.identifierApplied And Environmental Microbiology. Amer Soc Microbiology, v. 77, n. 18, n. 6357, n. 6367, 2011.
dc.identifier0099-2240
dc.identifierWOS:000294691400006
dc.identifier10.1128/AEM.05203-11
dc.identifierhttp://www.repositorio.unicamp.br/jspui/handle/REPOSIP/71220
dc.identifierhttp://repositorio.unicamp.br/jspui/handle/REPOSIP/71220
dc.identifier.urihttp://repositorioslatinoamericanos.uchile.cl/handle/2250/1289317
dc.descriptionCandida albicans and mutans streptococci are frequently detected in dental plaque biofilms from toddlers afflicted with early childhood caries. Glucosyltransferases (Gtfs) secreted by Streptococcus mutans bind to saliva-coated apatite (sHA) and to bacterial surfaces, synthesizing exopolymers in situ, which promote cell clustering and adherence to tooth enamel. We investigated the potential role Gtfs may play in mediating the interactions between C. albicans SC5314 and S. mutans UA159, both with each other and with the sHA surface. GtfB adhered effectively to the C. albicans yeast cell surface in an enzymatically active form, as determined by scintillation spectroscopy and fluorescence imaging. The glucans formed on the yeast cell surface were more susceptible to dextranase than those synthesized in solution or on sHA and bacterial cell surfaces (P < 0.05), indicating an elevated alpha-1,6-linked glucose content. Fluorescence imaging revealed that larger numbers of S. mutans cells bound to C. albicans cells with glucans present on their surface than to yeast cells without surface glucans (uncoated). The glucans formed in situ also enhanced C. albicans interactions with sHA, as determined by a novel single-cell micromechanical method. Furthermore, the presence of glucan-coated yeast cells significantly increased the accumulation of S. mutans on the sHA surface (versus S. mutans incubated alone or mixed with uncoated C. albicans; P < 0.05). These data reveal a novel cross-kingdom interaction that is mediated by bacterial GtfB, which readily attaches to the yeast cell surface. Surface-bound GtfB promotes the formation of a glucan-rich matrix in situ and may enhance the accumulation of S. mutans on the tooth enamel surface, thereby modulating the development of virulent biofilms.
dc.description77
dc.description18
dc.description6357
dc.description6367
dc.descriptionNIH [T32 DE07202-20]
dc.descriptionNIH [T32 DE07202-20]
dc.languageen
dc.publisherAmer Soc Microbiology
dc.publisherWashington
dc.publisherEUA
dc.relationApplied And Environmental Microbiology
dc.relationAppl. Environ. Microbiol.
dc.rightsaberto
dc.sourceWeb of Science
dc.subjectEarly-childhood Caries
dc.subjectExperimental Salivary Pellicle
dc.subjectGlucan-binding Proteins
dc.subjectBiofilm Formation
dc.subjectOral Streptococci
dc.subjectCell-wall
dc.subjectIn-vivo
dc.subjectAdherence
dc.subjectAdhesion
dc.subjectSucrose
dc.titleRole of Glucosyltransferase B in Interactions of Candida albicans with Streptococcus mutans and with an Experimental Pellicle on Hydroxyapatite Surfaces
dc.typeArtículos de revistas


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