Immunohistochemical and functional characterization of nitric oxide signaling pathway in isolated aorta from Crotalus durissus terrificus

dc.creatorMonica, FZ
dc.creatorRojas-Moscoso, J
dc.creatorPorto, M
dc.creatorSchenka, AA
dc.creatorAntunes, E
dc.creatorCogo, JC
dc.creatorDe Nucci, G
dc.date2012
dc.dateAPR
dc.date2014-07-30T18:05:55Z
dc.date2015-11-26T17:48:55Z
dc.date2014-07-30T18:05:55Z
dc.date2015-11-26T17:48:55Z
dc.date.accessioned2018-03-29T00:31:55Z
dc.date.available2018-03-29T00:31:55Z
dc.identifierComparative Biochemistry And Physiology C-toxicology & Pharmacology. Elsevier Science Inc, v. 155, n. 3, n. 433, n. 439, 2012.
dc.identifier1532-0456
dc.identifierWOS:000301312600001
dc.identifier10.1016/j.cbpc.2011.11.003
dc.identifierhttp://www.repositorio.unicamp.br/jspui/handle/REPOSIP/69950
dc.identifierhttp://repositorio.unicamp.br/jspui/handle/REPOSIP/69950
dc.identifier.urihttp://repositorioslatinoamericanos.uchile.cl/handle/2250/1289252
dc.descriptionWe characterized the nitric oxide (NO)-cyclic GMP-phosphodiesterase-5 (PDE5) pathway in Crotalus durissus terrificus aorta. Concentration responses curves to acetylcholine (ACh), sodium nitroprusside (SNP), BAY41-2272 (soluble guanylyl cyclase [sGC] stimulator), BAY60-2770 (sGC activator) and tadalafil (PDE5 inhibitor) were constructed in phenylephrine (10 mu M)-precontracted tissues with intact (E+) or denuded (E-) endothelium. ACh (0.0001-10 mu M) and SNP (0.0001-10 mu M) relaxed aorta, which were reduced by the NO synthase (L-NAME,100 mu M) or the sGC inhibitors (ODQ 10 mu M). Tadalafil (0.0001-10 mu M) relaxed E+ rings with potency (pEC(50)) and maximal response (E-max) values of 7.34 +/- 0.02 and 105 +/- 8%, respectively. E- or ODQ treatment significantly (P < 0.05) reduced tadalafil relaxations (66 +/- 18% and 71 +/- 7%, respectively). BAY41-2272 (0.0001-300 nM) produced concentration-dependent relaxations in E+ rings, which were reduced by addition of either ODQ or L-NAME (16.0- and 5.2-fold rightward shifts, respectively). The relaxation of BAY60-2770 was markedly potentiated by ODQ and L-NAME (41.0- and 9.7-fold leftward shifts, respectively), whereas in E- the pEC(50) values were shifted by 7-fold to the right. Immunohistochemistry, followed validation by transcriptomic analysis, revealed the presence of eNOS in endothelium, whereas nNOS was observed only in perivascular nerves. sGC and PDE5 were expressed in smooth muscle. Thus, NO-sGC-PDE5 pathway is evolutionarily present in Crotalus sp. vessels, and has a remarkable degree of functional similarity to mammalian vessels. (C) 2011 Elsevier Inc. All rights reserved.
dc.description155
dc.description3
dc.description433
dc.description439
dc.languageen
dc.publisherElsevier Science Inc
dc.publisherNew York
dc.publisherEUA
dc.relationComparative Biochemistry And Physiology C-toxicology & Pharmacology
dc.relationComp. Biochem. Physiol. C-Toxicol. Pharmacol.
dc.rightsfechado
dc.rightshttp://www.elsevier.com/about/open-access/open-access-policies/article-posting-policy
dc.sourceWeb of Science
dc.subjectAorta
dc.subjectCrotalus durissus
dc.subjectNitric oxide synthase
dc.subjectPhosphodiesterase type 5
dc.subjectSoluble guanylyl cyclase
dc.subjectSoluble-guanylate-cyclase
dc.subjectCyclic-nucleotide Phosphodiesterases
dc.subjectIndependent Activation
dc.subjectRabbit Aorta
dc.subjectBay 60-2770
dc.subjectRelaxation
dc.subjectAcetylcholine
dc.subjectEndothelium
dc.subjectInhibition
dc.subjectMechanisms
dc.titleImmunohistochemical and functional characterization of nitric oxide signaling pathway in isolated aorta from Crotalus durissus terrificus
dc.typeArtículos de revistas


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