Restriction enzyme analysis of DNA methylation in "condensed" chromatin of Ha-ras-transformed NIH 3T3 cells

dc.creatorMello, MLS
dc.creatorChambers, AF
dc.creatorVidal, BC
dc.creatorPlanding, W
dc.creatorSchenck, U
dc.date2000
dc.date2014-07-30T18:27:07Z
dc.date2015-11-26T17:48:25Z
dc.date2014-07-30T18:27:07Z
dc.date2015-11-26T17:48:25Z
dc.date.accessioned2018-03-29T00:31:18Z
dc.date.available2018-03-29T00:31:18Z
dc.identifierAnalytical Cellular Pathology. Ios Press, v. 20, n. 4, n. 163, n. 171, 2000.
dc.identifier0921-8912
dc.identifierWOS:000166980200003
dc.identifierhttp://www.repositorio.unicamp.br/jspui/handle/REPOSIP/71069
dc.identifierhttp://repositorio.unicamp.br/jspui/handle/REPOSIP/71069
dc.identifier.urihttp://repositorioslatinoamericanos.uchile.cl/handle/2250/1289088
dc.descriptionIncreased amounts of chromatin condensation (i.e., localized areas of high DNA density, or chromatin higher order packing state) have been described in NIH 3T3 cells transformed with the Ha-ras oncogene. The structural basis for this oncogene-mediated alteration in nuclear organization is unknown. Since DNA methylation is likely to be involved in regulating the nucleosomal level of DNA packaging, we studied the role of DNA methylation in higher-order chromatin organization induced by Ha-ras. CpG-methylated DNA content was estimated in "condensed" chromatin of Ha-ras-transformed NIH 3T3 cell lines which differ in ras expression and ras-induced metastatic ability but present approximately the same values of "condensed" chromatin areas. The question posed was that if DNA methylation were involved with the chromatin higher-order organization induced by Ha-ras in these cell lines, the methylated DNA density in the "condensed" chromatin would also be the same. The DNA evaluation was performed by video image analysis in Feulgen-stained cells previously subjected to treatment with Msp I and Hpa II restriction enzymes, which distinguish between methylated and non-methylated DNA. The amount of methylated CpG sequences not digested by Hpa II in "condensed" chromatin regions was found to vary in the studied ras-transformed cell lines. DNA CpG methylation status is thus suggested not to be involved with the higher order chromatin condensation induced by ras transformation in the mentioned NIH 3T3 cell lines.
dc.description20
dc.description4
dc.description163
dc.description171
dc.languageen
dc.publisherIos Press
dc.publisherAmsterdam
dc.publisherHolanda
dc.relationAnalytical Cellular Pathology
dc.relationAnal. Cell. Pathol.
dc.rightsfechado
dc.rightshttp://www.iospress.nl/service/authors/author-copyright-agreement/
dc.sourceWeb of Science
dc.subjectchromatin higher-order condensation
dc.subjectHa-ras
dc.subjectDNA methylation
dc.subjectrestriction endonucleases
dc.subjectimage analysis
dc.subjectBreast Epithelial-cells
dc.subjectImage-analysis
dc.subjectMetastatic Ability
dc.subjectNih-3t3 Fibroblasts
dc.subjectSignaling Pathway
dc.subjectP21 Expression
dc.subjectNuclear
dc.subjectOncogene
dc.subjectEndonucleases
dc.subjectChromosomes
dc.titleRestriction enzyme analysis of DNA methylation in "condensed" chromatin of Ha-ras-transformed NIH 3T3 cells
dc.typeArtículos de revistas


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