Inhibition of reverse transcriptase activity of HIV by polysaccharides of brown algae

dc.creatorQueiroz, KCS
dc.creatorMedeiros, VP
dc.creatorQueiroz, LS
dc.creatorAbreu, LRD
dc.creatorRocha, HAO
dc.creatorFerreira, CV
dc.creatorJuca, MB
dc.creatorAoyama, H
dc.creatorLeite, EL
dc.date2008
dc.dateJUN
dc.date2014-11-18T07:52:28Z
dc.date2015-11-26T17:47:56Z
dc.date2014-11-18T07:52:28Z
dc.date2015-11-26T17:47:56Z
dc.date.accessioned2018-03-29T00:30:41Z
dc.date.available2018-03-29T00:30:41Z
dc.identifierBiomedicine & Pharmacotherapy. Elsevier France-editions Scientifiques Medicales Elsevier, v. 62, n. 5, n. 303, n. 307, 2008.
dc.identifier0753-3322
dc.identifierWOS:000257255100004
dc.identifier10.1016/j.biopha.2008.03.006
dc.identifierhttp://www.repositorio.unicamp.br/jspui/handle/REPOSIP/80632
dc.identifierhttp://www.repositorio.unicamp.br/handle/REPOSIP/80632
dc.identifierhttp://repositorio.unicamp.br/jspui/handle/REPOSIP/80632
dc.identifier.urihttp://repositorioslatinoamericanos.uchile.cl/handle/2250/1288934
dc.descriptionBrown algae have two kinds of acid polysaccharides present in the extracellular matrix: sulfated fucan and alginic acid. We have previously isolated and characterized fucans from several species of brown seaweed. The characterized fucans from Dictyotaceae are heterofucans containing mainly fucose, galactose, glucose, xylose, and/or uronic acid. The fucan from Fucus vesiculosus is a homofucan containing only sulfated fucose. We assessed the activity of these fucans as inhibitors of HIV from reverse transcriptase (RT). Using activated DNA and template primers poly(rA)-oligo(dT), we found that fucans at a concentration of 0.5-1.0 mu g/mL had a pronounced inhibitory effect in vitro on the avian reverse transcriptase, with the exception of xylogalactofucan isolated from Spatoglossum schroederi, which had no inhibitory activity. The alginic acid (1.0 mu g/mL) inhibited the reverse transcriptase activity by 51.1% using activated DNA. The inhibitory effect of fucans was eliminated by their desulfation. Furthermore, only xylofucoglucuronan from S. schroederi lost its activity after carboxyreduction. We suggest that fucan activity is not only dependent on the ionic changes but also on the sugar rings that act to spatially orientate the charges in a configuration that recognizes the enzyme, thus determining the specificity of the binding. (C) 2008 Elsevier Masson SAS. All rights reserved.
dc.description62
dc.description5
dc.description303
dc.description307
dc.languageen
dc.publisherElsevier France-editions Scientifiques Medicales Elsevier
dc.publisherParis
dc.publisherFrança
dc.relationBiomedicine & Pharmacotherapy
dc.relationBiomed. Pharmacother.
dc.rightsfechado
dc.rightshttp://www.elsevier.com/about/open-access/open-access-policies/article-posting-policy
dc.sourceWeb of Science
dc.subjectreverse transcriptase
dc.subjectfucans
dc.subjectalgae
dc.subjectalginic acid
dc.subjectfucoidan
dc.subjectsulfated polysaccharides
dc.subjectSpatoglossum-schroederi
dc.subjectAnticoagulant Activity
dc.subjectSulfated Fucans
dc.subjectInfection
dc.subjectHeparin
dc.subjectAcid
dc.titleInhibition of reverse transcriptase activity of HIV by polysaccharides of brown algae
dc.typeArtículos de revistas


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