Effects of enamel matrix derivative and transforming growth factor-beta 1 on human periodontal ligament fibroblasts

dc.creatorRodrigues, TLS
dc.creatorMarchesan, JT
dc.creatorColetta, RD
dc.creatorNovaes, AB
dc.creatorGrisi, MFD
dc.creatorSouza, SLS
dc.creatorTaba, M
dc.creatorPalioto, DB
dc.date2007
dc.dateJUN
dc.date2014-11-18T07:24:11Z
dc.date2015-11-26T17:47:47Z
dc.date2014-11-18T07:24:11Z
dc.date2015-11-26T17:47:47Z
dc.date.accessioned2018-03-29T00:30:30Z
dc.date.available2018-03-29T00:30:30Z
dc.identifierJournal Of Clinical Periodontology. Blackwell Publishing, v. 34, n. 6, n. 514, n. 522, 2007.
dc.identifier0303-6979
dc.identifierWOS:000246577600009
dc.identifier10.1111/j.1600-051X.2007.01090.x
dc.identifierhttp://www.repositorio.unicamp.br/jspui/handle/REPOSIP/64155
dc.identifierhttp://www.repositorio.unicamp.br/handle/REPOSIP/64155
dc.identifierhttp://repositorio.unicamp.br/jspui/handle/REPOSIP/64155
dc.identifier.urihttp://repositorioslatinoamericanos.uchile.cl/handle/2250/1288888
dc.descriptionAim: The objective of this study was to evaluate the effects of enamel matrix derivative (EMD), transforming growth factor-beta 1 (TGF-beta 1), and a combination of both factors (EMD+TGF-beta 1) on periodontal ligament (PDL) fibroblasts. Material and methods: Human PDL fibroblasts were obtained from three adult patients with a clinically healthy periodontium, using the explant technique. The effects of EMD, TGF-beta 1, or a combination of both were analysed on PDL cell proliferation, adhesion, wound healing, and total protein synthesis, and on alkaline phosphatase (ALP) activity and bone-like nodule formation. Results: Treatment with EMD for 4, 7, and 10 days increased cell proliferation significantly compared with the negative control (p < 0.05). At day 10, EMD and EMD+TGF-beta 1 showed a higher cell proliferation compared with TGF-beta 1 (p < 0.01). Cell adhesion was significantly up-regulated by TGF-beta 1 compared with EMD and EMD+TGF-beta 1 (p < 0.01). EMD enhanced in vitro wound healing of PDL cells compared with the other treatments. Total protein synthesis was significantly increased in PDL cells cultured with EMD compared with PDL cells treated with TGF-beta 1 or EMD+TGF-beta 1 (p < 0.05). EMD induced ALP activity in PDL fibroblasts, which was associated with an increase of bone-like nodules. Conclusion: These findings support the hypothesis that EMD and TGF-beta 1 may play an important role in periodontal regeneration. EMD induced PDL fibroblast proliferation and migration, total protein synthesis, ALP activity, and mineralization, while TGF-beta 1 increased cellular adhesion. However, the combination of both factors did not positively alter PDL fibroblast behaviour.
dc.description34
dc.description6
dc.description514
dc.description522
dc.languageen
dc.publisherBlackwell Publishing
dc.publisherOxford
dc.publisherInglaterra
dc.relationJournal Of Clinical Periodontology
dc.relationJ. Clin. Periodontol.
dc.rightsfechado
dc.sourceWeb of Science
dc.subjectenamel matrix derivative
dc.subjectfibroblasts
dc.subjectgrowth factors
dc.subjectperiodontal
dc.subjectperiodontal ligament
dc.subjectTGF-beta 1
dc.subjectGrowth-factor-beta
dc.subjectIn-vitro
dc.subjectGingival Fibroblasts
dc.subjectFactors Invitro
dc.subjectCells
dc.subjectProliferation
dc.subjectRegeneration
dc.subjectTgf-beta-1
dc.subjectAttachment
dc.subjectCombination
dc.titleEffects of enamel matrix derivative and transforming growth factor-beta 1 on human periodontal ligament fibroblasts
dc.typeArtículos de revistas


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