IN VITRO HEMOLYTIC ACTIVITY OF Bothrops lanceolatus (FER-DE-LANCE) VENOM

dc.creatorMartins, LJ
dc.creatorde Araujo, PMF
dc.creatorBon, C
dc.creatorHyslop, S
dc.creatorde Araujo, AL
dc.date2009
dc.date2014-07-30T18:02:05Z
dc.date2015-11-26T17:47:37Z
dc.date2014-07-30T18:02:05Z
dc.date2015-11-26T17:47:37Z
dc.date.accessioned2018-03-29T00:30:18Z
dc.date.available2018-03-29T00:30:18Z
dc.identifierJournal Of Venomous Animals And Toxins Including Tropical Diseases. Cevap-unesp, v. 15, n. 3, n. 498, n. 508, 2009.
dc.identifier1678-9199
dc.identifierWOS:000270417000011
dc.identifierhttp://www.repositorio.unicamp.br/jspui/handle/REPOSIP/69388
dc.identifierhttp://repositorio.unicamp.br/jspui/handle/REPOSIP/69388
dc.identifier.urihttp://repositorioslatinoamericanos.uchile.cl/handle/2250/1288837
dc.descriptionBothrops lanceolatus venom contains a variety of enzymatic and biological activities. The present work investigated the hemolytic activity of this venom and its phospholipase A(2) (PLA(2)). Bothrops lanceolatus venom (6.7 mu g/mL) caused indirect hemolysis of cow, horse, rat and sheep erythrocytes, with horse erythrocytes being the most sensitive; no direct hemolysis was observed. Hemolysis in sheep erythrocytes was concentration-dependent (5-11.7 mu g/mL) and markedly attenuated by heating the venom for 30 minutes at >= 40 degrees C and by the PLA(2) inhibitor p-bromophenacyl bromide. An acidic PLA(2) (5 mu g/mL) purified from B. lanceolatus venom also caused hemolysis. This PLA(2) showed immunoprecipitin lines with antivenom against B. lanceolatus, which suggests that the enzymatic and hemolytic activities of this enzyme may be neutralized during antivenom therapy. These results indicate that B. lanceolatus venom and its PLA(2) can cause hemolysis in vitro.
dc.description15
dc.description3
dc.description498
dc.description508
dc.languageen
dc.publisherCevap-unesp
dc.publisherBotucatu
dc.publisherBrasil
dc.relationJournal Of Venomous Animals And Toxins Including Tropical Diseases
dc.relationJ. Venom. Anim. Toxins Trop. Dis.
dc.rightsaberto
dc.sourceWeb of Science
dc.subjectBothrops lanceolatus
dc.subjecthemolytic activity
dc.subjectphospholipase A(2)
dc.subjectsnake venom
dc.subjectAcidic Phospholipase A(2)
dc.subjectSnake-venom
dc.subjectCosta-rica
dc.subjectPharmacological-properties
dc.subjectNeutralizing Potency
dc.subjectChoco Colombia
dc.subjectAntivenom
dc.subjectPurification
dc.subjectAsper
dc.subjectMartinique
dc.titleIN VITRO HEMOLYTIC ACTIVITY OF Bothrops lanceolatus (FER-DE-LANCE) VENOM
dc.typeArtículos de revistas


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