Participation of leptin in the determination of the macrophage phenotype: an additional role in adipocyte and macrophage crosstalk

dc.creatorAcedo, SC
dc.creatorGambero, S
dc.creatorCunha, FGP
dc.creatorLorand-Metze, I
dc.creatorGambero, A
dc.date2013
dc.dateJUN
dc.date2014-07-30T14:30:46Z
dc.date2015-11-26T17:47:36Z
dc.date2014-07-30T14:30:46Z
dc.date2015-11-26T17:47:36Z
dc.date.accessioned2018-03-29T00:30:17Z
dc.date.available2018-03-29T00:30:17Z
dc.identifierIn Vitro Cellular & Developmental Biology-animal. Springer, v. 49, n. 6, n. 473, n. 478, 2013.
dc.identifier1071-2690
dc.identifierWOS:000320279500011
dc.identifier10.1007/s11626-013-9629-x
dc.identifierhttp://www.repositorio.unicamp.br/jspui/handle/REPOSIP/59096
dc.identifierhttp://repositorio.unicamp.br/jspui/handle/REPOSIP/59096
dc.identifier.urihttp://repositorioslatinoamericanos.uchile.cl/handle/2250/1288831
dc.descriptionFundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)
dc.descriptionMacrophages develop into specialized cell types with special functional properties, depending on locally produced stimuli. Adipose tissue macrophages present particular characteristics, such as the M2 cell phenotype, and produce cytokines and chemokines usually produced by M1 cells. Our aim was to study the role of leptin, which is an adipokine produced and released by adipocytes, in the induction of these characteristics in macrophages found in the adipose tissue. Human CD14(+) cells were obtained and maintained in culture with IFN-gamma (classical M1 phenotype), IL-4 (alternative M2 phenotype) or leptin for 5 d. Surface marker expression was then analyzed by cytometry. In addition, the release of tumor necrosis factor alpha (TNF-alpha), interleukin (IL)-6, IL-1 beta, IL-10, IL-1ra, MCP-1, MIP-1 alpha, and RANTES was quantified by ELISA after an LPS stimulus, in the culture supernatant. Macrophages exposed to leptin in culture expressed surface markers that were more similar to the M2 phenotype, but they were able to produce TNF-alpha, IL-6, IL-1 beta, IL-1ra, IL-10, MCP-1, and MIP-1 alpha, as observed for M1 cells. Results suggest that leptin strongly contributes to the phenotype observed in macrophages found in adipose tissue.
dc.description49
dc.description6
dc.description473
dc.description478
dc.descriptionFundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)
dc.descriptionFundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)
dc.languageen
dc.publisherSpringer
dc.publisherNew York
dc.publisherEUA
dc.relationIn Vitro Cellular & Developmental Biology-animal
dc.relationIn Vitro Cell. Dev. Biol.-Anim.
dc.rightsfechado
dc.rightshttp://www.springer.com/open+access/authors+rights?SGWID=0-176704-12-683201-0
dc.sourceWeb of Science
dc.subjectM1 phenotype
dc.subjectM2 phenotype
dc.subjectIFN-gamma
dc.subjectIL-4
dc.subjectAdipokine
dc.subjectAdipose-tissue Macrophages
dc.subjectFree Fatty-acids
dc.subjectMonocytes
dc.subjectObesity
dc.subjectMice
dc.subjectPolarization
dc.subjectActivation
dc.subjectExpression
dc.subjectPathways
dc.subjectReceptor
dc.titleParticipation of leptin in the determination of the macrophage phenotype: an additional role in adipocyte and macrophage crosstalk
dc.typeArtículos de revistas


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