In vitro drug release and ex vivo percutaneous absorption of resveratrol cream using HPLC with zirconized silica stationary phase

dc.creatorPolonini, HC
dc.creatorBastos, CD
dc.creatorde Oliveira, MAL
dc.creatorda Silva, CGA
dc.creatorCollins, CH
dc.creatorBrandao, MAF
dc.creatorRaposo, NRB
dc.date2014
dc.dateFEB 1
dc.date2014-07-30T18:02:02Z
dc.date2015-11-26T17:47:21Z
dc.date2014-07-30T18:02:02Z
dc.date2015-11-26T17:47:21Z
dc.date.accessioned2018-03-29T00:29:58Z
dc.date.available2018-03-29T00:29:58Z
dc.identifierJournal Of Chromatography B-analytical Technologies In The Biomedical And Life Sciences. Elsevier Science Bv, v. 947, n. 23, n. 31, 2014.
dc.identifier1570-0232
dc.identifier1873-376X
dc.identifierWOS:000331509100004
dc.identifier10.1016/j.jchromb.2013.12.005
dc.identifierhttp://www.repositorio.unicamp.br/jspui/handle/REPOSIP/69367
dc.identifierhttp://repositorio.unicamp.br/jspui/handle/REPOSIP/69367
dc.identifier.urihttp://repositorioslatinoamericanos.uchile.cl/handle/2250/1288755
dc.descriptionConselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)
dc.descriptionFundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)
dc.descriptionCoordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)
dc.descriptionSince the designs of optimal formulations for resveratrol permeation via the skin are lacking, the aim of this study was to establish the profile of resveratrol permeability into and across human skin. For that, a laboratory-made chromatographic column was used (Zr-PMODS), with its performance being compared to a traditional C18 column. In vitro drug release was conducted with polysulfone membranes, and the flux (J(s)) was 30.49 mu g cm(-2) h(-1)), with a lag time (L-T) of 0.04h, following a pseudo-first-order kinetics. For ex vivo percutaneous absorption using excised female human skin, the kinetic profile was the same, but J(s) was 0.87 mu g cm(-2) h(-1) and L-T was 0.97 h. From the initials 49.30 mu g applied to the skin, 9.50 mu g were quantified in the receptor medium, 20.48 mu g was retained at the stratum corneum (do not account as permeated) and 21.41 mu g was retained at the viable epidermis + dermis (account as permeated), totalizing 30.90 mu g of resveratrol permeated after 24 h of application (62.6%). From these results, one can conclude that a person using the 1-g emulsion dose released by the pump containing 20 mg of resveratrol will have, theoretically, 12.53 mg of it liberated into his bloodstream, gradually and continuously for 24h. (C) 2013 Elsevier B.V. All rights reserved.
dc.description947
dc.description23
dc.description31
dc.descriptionConselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)
dc.descriptionFundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)
dc.descriptionFundação de Amparo à Pesquisa do Estado de Minas Gerais (FAPEMIG)
dc.descriptionCoordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)
dc.descriptionPrograma Ciencia sem Fronteiras [245781/2012-9]
dc.descriptionConselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)
dc.descriptionFundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)
dc.descriptionCoordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)
dc.descriptionCAPES [Edital 04/CII-2008]
dc.descriptionPrograma Ciencia sem Fronteiras [245781/2012-9]
dc.languageen
dc.publisherElsevier Science Bv
dc.publisherAmsterdam
dc.publisherHolanda
dc.relationJournal Of Chromatography B-analytical Technologies In The Biomedical And Life Sciences
dc.relationJ. Chromatogr. B
dc.rightsfechado
dc.rightshttp://www.elsevier.com/about/open-access/open-access-policies/article-posting-policy
dc.sourceWeb of Science
dc.subjectIn vitro drug release
dc.subjectPercutaneous absorption
dc.subjectTransdermal dosage form
dc.subjectResveratrol
dc.subjectFactorial design
dc.subjectValidation studies
dc.subjectPerformance Liquid-chromatography
dc.subjectTrans-resveratrol
dc.subjectFactorial Design
dc.subjectSkin
dc.subjectDiffusion
dc.subjectCancer
dc.subjectMice
dc.titleIn vitro drug release and ex vivo percutaneous absorption of resveratrol cream using HPLC with zirconized silica stationary phase
dc.typeArtículos de revistas


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