IL4 and IFNalpha generation of dendritic cells reveals great migratory potential and NFkB and cJun expression in IL4DCs

dc.creatorde Azevedo, MTA
dc.creatorSaad, STO
dc.creatorGilli, SCO
dc.date2013
dc.date2014-07-30T18:00:26Z
dc.date2015-11-26T17:46:22Z
dc.date2014-07-30T18:00:26Z
dc.date2015-11-26T17:46:22Z
dc.date.accessioned2018-03-29T00:28:54Z
dc.date.available2018-03-29T00:28:54Z
dc.identifierImmunological Investigations. Informa Healthcare, v. 42, n. 8, n. 711, n. 725, 2013.
dc.identifier0882-0139
dc.identifierWOS:000325402800004
dc.identifier10.3109/08820139.2013.809580
dc.identifierhttp://www.repositorio.unicamp.br/jspui/handle/REPOSIP/69213
dc.identifierhttp://repositorio.unicamp.br/jspui/handle/REPOSIP/69213
dc.identifier.urihttp://repositorioslatinoamericanos.uchile.cl/handle/2250/1288469
dc.descriptionFundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)
dc.descriptionDendritic cells (DCs) recently revealed as a potent tumor vaccine component, are commonly differentiated from monocytes by cultivation with IL-4 and GM-CSF. Despite the different opinions, the use of IFNalpha can promote DCs differentiation and activation. The aim of this study was to compare the functionality and phenotypic characterization of monocyte-derived DC generated by IL-4 (IL4DC) and IFNalpha (IFNalphaDC) modified protocols. To this aim, we investigated the expression of maturation markers, co-stimulatory molecules, relevant miRNA, cytokine and migratory profiles and the functional ability of these cells to stimulate autologous T cells in vitro. We herein investigated the molecular mechanism underlying the parameters previously described, as the relative expression of NF-kB p65, c-fos and c-jun, transcription factors. Our results demonstrated that IL4DC presented a stable phenotype, an increase in migratory capacity and NF-KB activation, in addition to lower levels of miR-146 a and miR-221. We believe that the IL4DC migratory potential and increase in NFkBp65 expression may be involved in higher IL12 expression and migration, suggesting a preferential activation of TH1 immune responses by IL4DC.
dc.description42
dc.description8
dc.description711
dc.description725
dc.descriptionFundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)
dc.descriptionINCTS
dc.descriptionFundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)
dc.languageen
dc.publisherInforma Healthcare
dc.publisherLondon
dc.publisherInglaterra
dc.relationImmunological Investigations
dc.relationImmunol. Invest.
dc.rightsfechado
dc.rightshttp://informahealthcare.com/userimages/ContentEditor/1255620309227/Copyright_And_Permissions.pdf
dc.sourceWeb of Science
dc.subjectDendritic cells
dc.subjectIl4
dc.subjectIfnalpha
dc.subjectStimulatory Factor
dc.subjectImmune-responses
dc.subjectGamma Production
dc.subjectT-cells
dc.subjectMaturation
dc.subjectIl-12
dc.subjectInterferon
dc.subjectDistinct
dc.subjectActivation
dc.subjectInduction
dc.titleIL4 and IFNalpha generation of dendritic cells reveals great migratory potential and NFkB and cJun expression in IL4DCs
dc.typeArtículos de revistas


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