Electrophoresis and spectrometric analyses of adaptation-related proteins in thermally stressed Chromobacterium violaceum

dc.creatorCordeiro, IB
dc.creatorCastro, DP
dc.creatorNogueira, PPO
dc.creatorAngelo, PCS
dc.creatorNogueira, PA
dc.creatorGoncalves, JFC
dc.creatorPereira, AMRF
dc.creatorGarcia, JS
dc.creatorSouza, GHMF
dc.creatorArruda, MAZ
dc.creatorEberlin, MN
dc.creatorAstolfi, S
dc.creatorAndrade, EV
dc.creatorLopez-Lozano, JL
dc.date2013
dc.date2014-07-30T17:19:14Z
dc.date2015-11-26T17:46:12Z
dc.date2014-07-30T17:19:14Z
dc.date2015-11-26T17:46:12Z
dc.date.accessioned2018-03-29T00:28:42Z
dc.date.available2018-03-29T00:28:42Z
dc.identifierGenetics And Molecular Research. Funpec-editora, v. 12, n. 4, n. 5057, n. 5071, 2013.
dc.identifier1676-5680
dc.identifierWOS:000331608000095
dc.identifier10.4238/2013.October.29.1
dc.identifierhttp://www.repositorio.unicamp.br/jspui/handle/REPOSIP/64598
dc.identifierhttp://repositorio.unicamp.br/jspui/handle/REPOSIP/64598
dc.identifier.urihttp://repositorioslatinoamericanos.uchile.cl/handle/2250/1288418
dc.descriptionConselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)
dc.descriptionCoordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)
dc.descriptionChromobacterium violaceum is a Gram-negative proteobacteria found in water and soil; it is widely distributed in tropical and subtropical regions, such as the Amazon rainforest. We examined protein expression changes that occur in C. violaceum at different growth temperatures using electrophoresis and mass spectrometry. The total number of spots detected was 1985; the number ranged from 99 to 380 in each assay. The proteins that were identified spectrometrically were categorized as chaperones, proteins expressed exclusively under heat stress, enzymes involved in the respiratory and fermentation cycles, ribosomal proteins, and proteins related to transport and secretion. Controlling inverted repeat of chaperone expression and inverted repeat DNA binding sequences, as well as regions recognized by sigma factor 32, elements involved in the genetic regulation of the bacterial stress response, were identified in the promoter regions of several of the genes coding proteins, involved in the C. violaceum stress response. We found that 30 degrees C is the optimal growth temperature for C. violaceum, whereas 25, 35, and 40 degrees C are stressful temperatures that trigger the expression of chaperones, superoxide dismutase, a probable small heat shock protein, a probable phasing, ferrichrome-iron receptor protein, elongation factor P, and an ornithine carbamoyltransferase catabolite. This information improves our comprehension of the mechanisms involved in stress adaptation by C. violaceum.
dc.description12
dc.description4
dc.description5057
dc.description5071
dc.descriptionConselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)
dc.descriptionFAPEAM
dc.descriptionCoordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)
dc.descriptionFINEP
dc.descriptionConselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)
dc.descriptionCoordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)
dc.languageen
dc.publisherFunpec-editora
dc.publisherRibeirao Preto
dc.publisherBrasil
dc.relationGenetics And Molecular Research
dc.relationGenet. Mol. Res.
dc.rightsfechado
dc.sourceWeb of Science
dc.subjectCIRCE
dc.subjectChaperones
dc.subjectHeat shock response
dc.subjectHSP
dc.subjectDifferential gene expression
dc.subjectHeat-shock
dc.subjectEscherichia-coli
dc.subjectBacillus-subtilis
dc.subjectAcid
dc.subjectBacteria
dc.subjectGrowth
dc.subjectIdentification
dc.subjectTransporters
dc.subjectExpression
dc.subjectProteomics
dc.titleElectrophoresis and spectrometric analyses of adaptation-related proteins in thermally stressed Chromobacterium violaceum
dc.typeArtículos de revistas


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