Expression of hypoxia-inducible factor 1 alpha in mononuclear phagocytes infected with Leishmania amazonensis

dc.creatorDegrossoli, A
dc.creatorBosetto, MC
dc.creatorLima, CBC
dc.creatorGiorgio, S
dc.date2007
dc.dateDEC 15
dc.date2014-11-18T03:39:09Z
dc.date2015-11-26T17:45:56Z
dc.date2014-11-18T03:39:09Z
dc.date2015-11-26T17:45:56Z
dc.date.accessioned2018-03-29T00:28:24Z
dc.date.available2018-03-29T00:28:24Z
dc.identifierImmunology Letters. Elsevier Science Bv, v. 114, n. 2, n. 119, n. 125, 2007.
dc.identifier0165-2478
dc.identifierWOS:000252164600009
dc.identifier10.1016/j.imlet.2007.09.009
dc.identifierhttp://www.repositorio.unicamp.br/jspui/handle/REPOSIP/66477
dc.identifierhttp://www.repositorio.unicamp.br/handle/REPOSIP/66477
dc.identifierhttp://repositorio.unicamp.br/jspui/handle/REPOSIP/66477
dc.identifier.urihttp://repositorioslatinoamericanos.uchile.cl/handle/2250/1288341
dc.descriptionIncreasing evidence indicates that hypoxia-inducible factor 1 alpha (HIF-1 alpha) can be upregulated indifferent cell types by nonhypoxic stimuli such as growth factors, cytokines, nitric oxide, lipopolysaccharides and a range of infectious microorganisms. In this study, the ability of the following mononuclear phagocytes to express HIF-1 alpha is reported: mouse macrophages (mM Phi)), human macrophages (W(D) and human dendritic cells (DC), parasitized in vitro with Leishmania amazonensis; as assessed by immunofluorescence microscopy. A logical explanation for HIF-1 alpha expression might be that the mononuclear phagocytes became hypoxic after L. amazonensis infection. Using the hypoxia marker pimonidazole, observation revealed that L. amazonensis-infected cells were not hypoxic. In addition, experiments using a HIF-1 alpha inhibitor, CdCl2, to treat L. amazonensis-infected macrophage cultures showed reduced parasite survival. These studies indicated that HIF-1 alpha could play a role in adaptative and immune responses of mononuclear phagocytes presenting infection by the parasite L. amazonensis. (c) 2007 Elsevier B.V. All rights reserved.
dc.description114
dc.description2
dc.description119
dc.description125
dc.languageen
dc.publisherElsevier Science Bv
dc.publisherAmsterdam
dc.publisherHolanda
dc.relationImmunology Letters
dc.relationImmunol. Lett.
dc.rightsfechado
dc.rightshttp://www.elsevier.com/about/open-access/open-access-policies/article-posting-policy
dc.sourceWeb of Science
dc.subjecthypoxia-inducible factor 1 alpha
dc.subjectmononuclear phagocytes
dc.subjectLeishmania amazonensis
dc.subjectHypoxia-inducible Factor-1-alpha
dc.subjectHuman Dendritic Cells
dc.subjectGene-expression
dc.subjectFactor-i
dc.subjectIntracellular Parasites
dc.subjectHif-1-alpha Protein
dc.subjectToxoplasma-gondii
dc.subjectHuman Macrophages
dc.subjectFactor 1-alpha
dc.subjectNitric-oxide
dc.titleExpression of hypoxia-inducible factor 1 alpha in mononuclear phagocytes infected with Leishmania amazonensis
dc.typeArtículos de revistas


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