Identification of nuclear factor-kappa B sites in the Slc2a4 gene promoter

dc.creatorFuruya, DT
dc.creatorNeri, EA
dc.creatorPoletto, AC
dc.creatorAnhe, GF
dc.creatorFreitas, HS
dc.creatorCampello, RS
dc.creatorReboucas, NA
dc.creatorMachado, UF
dc.date2013
dc.dateMAY 6
dc.date2014-07-30T18:00:21Z
dc.date2015-11-26T17:45:52Z
dc.date2014-07-30T18:00:21Z
dc.date2015-11-26T17:45:52Z
dc.date.accessioned2018-03-29T00:28:18Z
dc.date.available2018-03-29T00:28:18Z
dc.identifierMolecular And Cellular Endocrinology. Elsevier Ireland Ltd, v. 370, n. 41671, n. 87, n. 95, 2013.
dc.identifier0303-7207
dc.identifierWOS:000319548200009
dc.identifier10.1016/j.mce.2013.01.019
dc.identifierhttp://www.repositorio.unicamp.br/jspui/handle/REPOSIP/69167
dc.identifierhttp://repositorio.unicamp.br/jspui/handle/REPOSIP/69167
dc.identifier.urihttp://repositorioslatinoamericanos.uchile.cl/handle/2250/1288317
dc.descriptionFundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)
dc.descriptionGlucose transporter GLUT4 protein, codified by Slc2a4 gene plays a key role in glycemic homeostasis. Insulin resistance, as in obesity, has been associated to inflammatory state, in which decreased GLUT4 is a feature. Inflammatory NF-kappa B transcriptional factor has been proposed as a repressor of Slc2a4; although, the binding site(s) in Slc2a4 promoter and the direct repressor effect have never been reported yet. A motif-based sequence analysis of mouse Slc2a4 promoter revealed two putative kappa B sites located inside -83/-62 and -134/-113 bp. Eletrophoretic mobility assay showed that p50 and p65 NF-kappa B subunits bind to both putative kappa B sites. Chromatin immunoprecipitation assay using genomic DNA from adipocytes confirmed p50- and p65-binding to Slc2a4 promoter. Moreover, transfection experiments revealed that NF-kappa B binds to the -134/-113 bp region of the mouse Slc2a4 gene promoter, inhibiting the Slc2a4 gene transcription. The current findings demonstrate the existence of two kappa B sites in Slc2a4 gene promote, and that NF-kappa B has a direct repressor effect upon the Slc2a4 gene, providing an important link between insulin resistance and inflammation. (C) 2013 Elsevier Ireland Ltd. All rights reserved.
dc.description370
dc.description41671
dc.description87
dc.description95
dc.descriptionFundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)
dc.descriptionFundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)
dc.descriptionFAPESP [07/50554-1, 08/09194-4, 11/08570-5]
dc.languageen
dc.publisherElsevier Ireland Ltd
dc.publisherClare
dc.publisherIrlanda
dc.relationMolecular And Cellular Endocrinology
dc.relationMol. Cell. Endocrinol.
dc.rightsfechado
dc.rightshttp://www.elsevier.com/about/open-access/open-access-policies/article-posting-policy
dc.sourceWeb of Science
dc.subjectNF-kappa B
dc.subjectSlc2a4
dc.subjectGLUT4
dc.subjectInsulin resistance
dc.subjectInflammation
dc.subjectGene reporter
dc.subjectNecrosis-factor-alpha
dc.subjectTreated Obese Mice
dc.subject3t3-l1 Adipocytes
dc.subjectInsulin-resistance
dc.subjectGlut4 Gene
dc.subjectExpression
dc.subjectActivation
dc.subjectElements
dc.titleIdentification of nuclear factor-kappa B sites in the Slc2a4 gene promoter
dc.typeArtículos de revistas


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