Polyploidy and chromatin remodeling in hepatocytes from insulin-dependent diabetic and normoglycemic aged mice

dc.creatorGhiraldini, FG
dc.creatorSilva, IS
dc.creatorMello, MLS
dc.date2012
dc.dateSEP
dc.date2014-07-30T18:09:56Z
dc.date2015-11-26T17:45:43Z
dc.date2014-07-30T18:09:56Z
dc.date2015-11-26T17:45:43Z
dc.date.accessioned2018-03-29T00:28:08Z
dc.date.available2018-03-29T00:28:08Z
dc.identifierCytometry Part A. Wiley-blackwell, v. 81A, n. 9, n. 755, n. 764, 2012.
dc.identifier1552-4922
dc.identifierWOS:000307839900006
dc.identifier10.1002/cyto.a.22102
dc.identifierhttp://www.repositorio.unicamp.br/jspui/handle/REPOSIP/70763
dc.identifierhttp://repositorio.unicamp.br/jspui/handle/REPOSIP/70763
dc.identifier.urihttp://repositorioslatinoamericanos.uchile.cl/handle/2250/1288269
dc.descriptionFundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)
dc.descriptionConselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)
dc.descriptionChanges in polyploidization, chromatin supraorganization, and chromatin accessibility were investigated in hepatocytes collected from adult, nonobese diabetic (NOD) mice with increasing hyperglycemia and compared with adult normoglycemic controls and 56-week-old normoglycemic BALB/c mice. Our goal was to determine the changes in ploidy degrees and chromatin characteristics in mouse hepatocytes that are associated with insulin-dependent diabetes and to detect similarities in these aspects with those verified with aging, with greater accuracy than previous studies. Image analysis of Feulgen-stained nuclei revealed changes in ploidy degrees and chromatin supraorganization. Chromatin accessibility was assessed with micrococcal nuclease (MNase) digestion. Increased polyploidy was associated with increasing levels of glycemia, and this trend toward polyploidy was found even under normoglycemic conditions in NOD mice. Although high degrees of ploidy were also detected in aged BALB/c mice, the magnitude of polyploidy was not the same magnitude as that in the diabetic mice. While there was increased homogeneity of chromatin packaging with increasing polyploidy under conditions of severe hyperglycemia (and even under conditions of normoglycemia) in NOD mice, an inverse relationship was observed in aged BALB/c mice. Chromatin accessibility to MNase increased under severe hyperglycemia and advanced age, but it was much higher in the diabetic mice. In conclusion, although similarities in polyploidy were observed between the hepatocytes from increasingly hyperglycemic adult mice and those from normoglycemic aged mice, the relationship between chromatin remodeling and increases in ploidy degrees was not the same between the hepatocytes of these two groups. These findings demonstrate that strict similarities between diabetes and aging are not always true at the cellular level. This discordance is likely due to differences in the metabolic state of mouse hepatocytes during aging and diabetic conditions consequent to specificities in their gene regulatory programs. (C) 2012 International Society for Advancement of Cytometry
dc.description81A
dc.description9
dc.description755
dc.description764
dc.descriptionFundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)
dc.descriptionConselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)
dc.descriptionFundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)
dc.descriptionConselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)
dc.descriptionFAPESP [2010/50015-6, 2008/58067-2]
dc.descriptionCNPq [471303/2009-7, 301943/2009-5, 506463/2010-9]
dc.languageen
dc.publisherWiley-blackwell
dc.publisherHoboken
dc.publisherEUA
dc.relationCytometry Part A
dc.relationCytom. Part A
dc.rightsfechado
dc.rightshttp://olabout.wiley.com/WileyCDA/Section/id-406071.html
dc.sourceWeb of Science
dc.subjectaging
dc.subjectdiabetes
dc.subjecthepatocytes
dc.subjectpolyploidy
dc.subjectchromatin supraorganization
dc.subjectimage analysis
dc.subjectMNase
dc.subjectNOD mice
dc.subjectDna Methylation
dc.subjectMouse Hepatocytes
dc.subjectGene-expression
dc.subjectHistone Modification
dc.subjectTrichostatin-a
dc.subjectImage-analysis
dc.subjectCell-function
dc.subjectRat-liver
dc.subjectGrowth
dc.subjectProliferation
dc.titlePolyploidy and chromatin remodeling in hepatocytes from insulin-dependent diabetic and normoglycemic aged mice
dc.typeArtículos de revistas


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