Identification of differentially transcribed genes in shrimp Litopenaeus vannamei exposed to osmotic stress and challenged with WSSV virus

dc.creatorGoncalves-Soares, D
dc.creatorSeiffert, WQ
dc.creatorSchlindwein, AD
dc.creatorToledo-Silva, G
dc.creatorZanette, J
dc.creatorMarques, MRF
dc.creatorBainy, ACD
dc.date2012
dc.dateMAR
dc.date2014-07-30T18:00:18Z
dc.date2015-11-26T17:45:43Z
dc.date2014-07-30T18:00:18Z
dc.date2015-11-26T17:45:43Z
dc.date.accessioned2018-03-29T00:28:07Z
dc.date.available2018-03-29T00:28:07Z
dc.identifierComparative Biochemistry And Physiology D-genomics & Proteomics. Elsevier Science Inc, v. 7, n. 1, n. 73, n. 81, 2012.
dc.identifier1744-117X
dc.identifierWOS:000299983000006
dc.identifier10.1016/j.cbd.2011.11.002
dc.identifierhttp://www.repositorio.unicamp.br/jspui/handle/REPOSIP/69146
dc.identifierhttp://repositorio.unicamp.br/jspui/handle/REPOSIP/69146
dc.identifier.urihttp://repositorioslatinoamericanos.uchile.cl/handle/2250/1288268
dc.descriptionCoordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)
dc.descriptionConselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)
dc.descriptionThe effects of hyposmotic stress and white spot syndrome virus (WSSV) challenge in expression was studied in the marine shrimp Litopenaeus vannamei. Messenger RNA from gills of shrimp submitted to osmotic stress was isolated to identify genes differentially expressed through the suppressive subtractive hybridization (SSH) method. Two subtractive libraries forward and two reverse were constructed to identify up and down-regulated genes under these conditions. About 192 clones were sequenced, of which 46 genes were identified. These genes encode proteins corresponding to a wide range of biological roles, including defense, cell signaling, electron transfer, cell proliferation and differentiation, apoptosis, intermediary metabolism, cytoskeleton and digestion. Among the identified genes, 19 were up-regulated and 27 were down-regulated in the animals kept at a lower ion concentration. We evaluated the expression of eight genes by RT-qPCR in shrimp submitted to hyposmotic conditions with and without WSSV challenge. The SSH enabled the identification of genes that are influenced by hyposmotic stress. A significant up-regulation was observed in lectin-C, QM, TGF beta inducible nuclear protein 1, ciclophilin, malate dehydrogenase, mitochondrial ATP synthase F chain and ATP synthase subunit 9 precursor transcripts. However, the expression of these genes in L. vannamei was not affected by WSSV infection both at isosmotic and hyposmotic conditions. (C) 2011 Elsevier Inc. All rights reserved.
dc.description7
dc.description1
dc.description73
dc.description81
dc.descriptionFINEP-Brazil [01070625-00/336-2007]
dc.descriptionCoordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)
dc.descriptionConselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)
dc.descriptionCoordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)
dc.descriptionConselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)
dc.descriptionFINEP-Brazil [01070625-00/336-2007]
dc.languageen
dc.publisherElsevier Science Inc
dc.publisherNew York
dc.publisherEUA
dc.relationComparative Biochemistry And Physiology D-genomics & Proteomics
dc.relationComp. Biochem. Physiol. D-Genomics Proteomics
dc.rightsfechado
dc.rightshttp://www.elsevier.com/about/open-access/open-access-policies/article-posting-policy
dc.sourceWeb of Science
dc.subjectSSH
dc.subjectGill
dc.subjectLitopenaeus vannamei
dc.subjectOsmotic stress
dc.subjectWSSV
dc.subjectqPCR
dc.subjectWhite-spot-syndrome
dc.subjectSuppression Subtractive Hybridization
dc.subjectC-type Lectin
dc.subjectLipopolysaccharide-binding Protein
dc.subjectPolymerase-chain-reaction
dc.subjectFresh-water Crayfish
dc.subjectBlack Tiger Shrimp
dc.subjectPenaeus-monodon
dc.subjectLow-salinity
dc.subjectCarbonic-anhydrase
dc.titleIdentification of differentially transcribed genes in shrimp Litopenaeus vannamei exposed to osmotic stress and challenged with WSSV virus
dc.typeArtículos de revistas


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