Comparison of immunoexpression of 2 antibodies for estrogen receptors (1D5 and 6F11) in breast carcinomas using different antigen retrieval and detection methods

dc.creatorVassallo, J
dc.creatorPinto, GA
dc.creatorAlvarenga, M
dc.creatorZeferino, LC
dc.creatorChagas, CA
dc.creatorMetze, K
dc.date2004
dc.dateJUN
dc.date2014-07-30T14:00:12Z
dc.date2015-11-26T17:44:36Z
dc.date2014-07-30T14:00:12Z
dc.date2015-11-26T17:44:36Z
dc.date.accessioned2018-03-29T00:26:47Z
dc.date.available2018-03-29T00:26:47Z
dc.identifierApplied Immunohistochemistry & Molecular Morphology. Lippincott Williams & Wilkins, v. 12, n. 2, n. 177, n. 182, 2004.
dc.identifier1062-3345
dc.identifierWOS:000221718400014
dc.identifierhttp://www.repositorio.unicamp.br/jspui/handle/REPOSIP/56255
dc.identifierhttp://repositorio.unicamp.br/jspui/handle/REPOSIP/56255
dc.identifier.urihttp://repositorioslatinoamericanos.uchile.cl/handle/2250/1287920
dc.descriptionThe importance of in situ immunodetection of hormone receptors for therapy planning and prognostic evaluation in patients with breast carcinoma is well established. Sensitive detection methods are of utmost importance, especially in poorly fixed tissues, which are not uncommon in routine pathologic practice. The purpose of the present study is to compare immunoexpression of estrogen receptors in 20 cases of invasive ductal carcinoma using two antibodies, ID5 and 6F11, and to verify the effect of different antigen retrieval solutions and detection systems. Immunoperoxidase was performed on paraffin sections using ID5 and 6F11 as primary antibodies. Heat-induced antigen retrieval was performed using citrate buffer (pH 6.0) or Tris-EDTA buffer (pH 8.9). Detection was achieved using the following systems: Envision, Envision Plus, and labeled streptavidinbiotin peroxidase complex. Reaction was semiquantified from 0 to 4. There were no differences between the two markers, 1D5 and 6F11, except when 6F11 was used with Envision and citrate buffer, in which case weaker reactivity was observed. Only in this combination (6F11/EnVision) was EDTA buffer significantly better than citrate. Labeled streptavidin-biotin peroxidase complex presented the best results, followed by EnVision Plus.
dc.description12
dc.description2
dc.description177
dc.description182
dc.languageen
dc.publisherLippincott Williams & Wilkins
dc.publisherPhiladelphia
dc.publisherEUA
dc.relationApplied Immunohistochemistry & Molecular Morphology
dc.relationAppl. Immunohistochem.
dc.rightsfechado
dc.sourceWeb of Science
dc.subjectbreast carcinoma
dc.subjectimmunohistochemistry
dc.subjectprognostic factors
dc.subjectpredictive factors
dc.subjectLigand-binding Assay
dc.subjectProgesterone-receptors
dc.subjectImmunohistochemical Evaluation
dc.subjectMonoclonal-antibodies
dc.subjectPredicting Response
dc.subjectEnzyme-immunoassay
dc.subjectTissue-sections
dc.subjectCancer
dc.subjectFixation
dc.subjectImmunoreactivity
dc.titleComparison of immunoexpression of 2 antibodies for estrogen receptors (1D5 and 6F11) in breast carcinomas using different antigen retrieval and detection methods
dc.typeArtículos de revistas


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