FMNL1 promotes proliferation and migration of leukemia cells

dc.creatorFavaro, P
dc.creatorTraina, F
dc.creatorMachado-Neto, JA
dc.creatorLazarini, M
dc.creatorLopes, MR
dc.creatorPereira, JKN
dc.creatorCosta, FF
dc.creatorInfante, E
dc.creatorRidley, AJ
dc.creatorSaad, STO
dc.date2013
dc.dateSEP
dc.date2014-07-30T17:38:43Z
dc.date2015-11-26T17:44:35Z
dc.date2014-07-30T17:38:43Z
dc.date2015-11-26T17:44:35Z
dc.date.accessioned2018-03-29T00:26:46Z
dc.date.available2018-03-29T00:26:46Z
dc.identifierJournal Of Leukocyte Biology. Federation Amer Soc Exp Biol, v. 94, n. 3, n. 503, n. 512, 2013.
dc.identifier0741-5400
dc.identifier1938-3673
dc.identifierWOS:000328839400014
dc.identifier10.1189/jlb.0113057
dc.identifierhttp://www.repositorio.unicamp.br/jspui/handle/REPOSIP/67355
dc.identifierhttp://repositorio.unicamp.br/jspui/handle/REPOSIP/67355
dc.identifier.urihttp://repositorioslatinoamericanos.uchile.cl/handle/2250/1287915
dc.descriptionConselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)
dc.descriptionFundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)
dc.descriptionFMNL1 regulates leukemic cell proliferation, clonogenicity, migration, and interaction with endothelial cells, supporting a potential role in leukemogenesis. The human FMNL1 is expressed predominantly in hematopoietic cells and has been described previously as overexpressed in hematopoietic malignancies. However, it is not known whether FMNL1 contributes to leukemogenesis. Here, we investigate the FMNL1 function using two different human leukemia models: Namalwa and K562 cell lines. FMNL1 depletion reduced cell proliferation and colony formation in both leukemic cell types, as well as a decrease in the tumor growth of FMNL1-depleted Namalwa cell xenografts. In addition, there was a decrease in migration and in TEM in FMNL1-depleted Namalwa cells. FMNL1 endogenously associates with Rac1, and FMNL1 silencing resulted in an increased Rac1 activity. The reduced migration observed in FMNL1-depleted cells was restored by inhibiting Rac activity. Our results indicate that FMNL1 stimulates leukemia cell proliferation as well as migration. This suggests that FMNL1 contributes to leukemogenesis and could act in part through Rac1 regulation.
dc.description94
dc.description3
dc.description503
dc.description512
dc.descriptionConselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)
dc.descriptionFundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)
dc.descriptionCancer Research UK
dc.descriptionDepartment of Health via a National Institute for Health Research (NIHR) Comprehensive Biomedical Research Centre Award
dc.descriptionSt Thomas' NHS Foundation Trust
dc.descriptionKing's College London
dc.descriptionKing's College Hospital NHS Foundation Trust
dc.descriptionConselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)
dc.descriptionFundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)
dc.languageen
dc.publisherFederation Amer Soc Exp Biol
dc.publisherBethesda
dc.publisherEUA
dc.relationJournal Of Leukocyte Biology
dc.relationJ. Leukoc. Biol.
dc.rightsfechado
dc.sourceWeb of Science
dc.subjectformins
dc.subjectleukemogenesis
dc.subjectcell motility
dc.subjecttransendothelial migration
dc.subjectRac1
dc.subjectGuanosine Triphosphatases Represent
dc.subjectMammalian Formins
dc.subjectMyeloid-leukemia
dc.subjectGene-expression
dc.subjectLymphoma Cells
dc.subjectCancer-cells
dc.subjectRac Gtpases
dc.subjectRho Gtpases
dc.subjectK562 Cells
dc.subjectProtein
dc.titleFMNL1 promotes proliferation and migration of leukemia cells
dc.typeArtículos de revistas


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